PKN3 anticorps (AA 2-889)

N° du produit ABIN7871827

Cet anticorps anti-PKN3 Polyclonal Lapin (ABIN7871827) détecte spécifiquement PKN3 dans WB, ELISA et FACS. L’anticorps est réactif avec des échantillons de Humain.

Aperçu rapide pour PKN3 anticorps (AA 2-889) (ABIN7871827)

Antigène: PKN3 (Protein Kinase N3 (PKN3))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp PKN3 est non-conjugé

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Détail du produit anti-PKN3 anticorps

Épitope: AA 2-889

Fonction: PKN3 Antibody / Protein kinase N3

Purification: Immunogen affinity purified

Immunogène: E.coli-derived human PKN3 recombinant protein (Position: E2-P889) was used as the immunogen for the PKN3 antibody.

Isotype: IgG

Information d'application

Indications d'application: Optimal dilution of the PKN3 antibody should be determined by the researcher.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Stock: 4 °C,-20 °C

Stockage commentaire: After reconstitution, the PKN3 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Détails sur PKN3

Antigène: PKN3 (Protein Kinase N3 (PKN3))

Autre désignation: PKN3

Sujet: PKN3 antibody detects Protein kinase N3, a serine/threonine kinase involved in cytoskeletal organization, cell adhesion, and tumorigenesis. Encoded by the PKN3 gene on chromosome 9q31.2, this enzyme is a member of the protein kinase N (PKN) family, which are effectors of the Rho family of small GTPases. PKN3 links Rho signaling to downstream pathways that regulate actin dynamics, cell migration, and proliferation, making it a key mediator of cytoskeletal remodeling and oncogenic transformation.

Structurally, PKN3 contains an N-terminal regulatory region with HR1 (homology region 1) motifs that bind Rho GTPases, followed by a C-terminal catalytic domain similar to protein kinase C (PKC). PKN3 phosphorylates substrates involved in actin filament stabilization and cell junction assembly, coordinating mechanical and adhesive cues. It is localized to the cytoplasm and plasma membrane, where it interacts with focal adhesion proteins and scaffolds to transmit signals influencing motility and growth.

The PKN3 antibody is widely used in cancer biology, cell signaling, and cytoskeletal research to investigate Rho-dependent kinase pathways and cell morphology control. Western blot analysis detects a 110 kilodalton band corresponding to PKN3, while immunofluorescence shows diffuse cytoplasmic and membrane-associated staining in epithelial and tumor cells. This antibody supports research into the molecular basis of migration, invasion, and metastatic progression.

PKN3 is overexpressed in several cancers, including breast, prostate, and pancreatic carcinomas, where it promotes invasive behavior through activation of the PI3K/AKT and RhoA pathways. Genetic or pharmacologic inhibition of PKN3 suppresses tumor growth and metastasis, making it a promising therapeutic target. Beyond oncology, PKN3 also contributes to endothelial cell barrier function and angiogenesis. The PKN3 antibody provides a valuable reagent for dissecting the cellular functions and therapeutic potential of this kinase. NSJ Bioreagents validates this antibody for its applications, ensuring consistent and reliable results for signal transduction research.

UniProt: Q6P5Z2