TRIM32 anticorps (AA 216-586)

N° du produit ABIN7872242

Cet anticorps Lapin Polyclonal détecte spécifiquement TRIM32 dans WB, IHC, ELISA et FACS. Il présente une réactivité avec des échantillons de Humain, Souris et Rat.

Aperçu rapide pour TRIM32 anticorps (AA 216-586) (ABIN7872242)

Antigène: TRIM32 (Tripartite Motif Containing 32 (TRIM32))

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp TRIM32 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Flow Cytometry (FACS)

Détail du produit anti-TRIM32 anticorps

Épitope: AA 216-586

Fonction: TRIM32 Antibody / Tripartite motif-containing protein 32

Purification: Immunogen affinity purified

Immunogène: E.coli-derived human TRIM32 recombinant protein (Position: S216-R586) was used as the immunogen for the TRIM32 antibody.

Isotype: IgG

Information d'application

Indications d'application: Optimal dilution of the TRIM32 antibody should be determined by the researcher.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Stock: 4 °C,-20 °C

Stockage commentaire: After reconstitution, the TRIM32 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Détails sur TRIM32

Antigène: TRIM32 (Tripartite Motif Containing 32 (TRIM32))

Autre désignation: TRIM32

Sujet: TRIM32 antibody detects Tripartite motif-containing protein 32, an E3 ubiquitin ligase that regulates protein turnover, muscle differentiation, and cellular signaling. TRIM32 is part of the TRIM family of RING finger proteins that mediate ubiquitin-dependent degradation and transcriptional regulation. The TRIM32 antibody is widely used in muscle biology, neurodegeneration, and oncology research to study ubiquitination, differentiation, and signaling regulation.

TRIM32 is encoded by the TRIM32 gene located on human chromosome 9q33.1. The protein is approximately 653 amino acids long and contains a RING domain, one or two B-box domains, and a coiled-coil region that mediates protein-protein interactions, followed by a C-terminal NHL repeat domain responsible for substrate recognition. TRIM32 localizes to the cytoplasm, nucleus, and occasionally to cytoskeletal structures, depending on cell type and activity state.

The TRIM32 antibody detects an 80 kilodalton protein by western blot and shows punctate cytoplasmic staining under immunofluorescence microscopy. TRIM32 functions as an E3 ubiquitin ligase that targets a variety of substrates involved in differentiation, including actin regulators, transcription factors, and signaling molecules. In skeletal muscle, TRIM32 promotes myogenesis by ubiquitinating inhibitors of differentiation such as NDRG2 and MyoD regulators. In neurons, TRIM32 modulates synaptic plasticity and axonal growth by regulating cytoskeletal remodeling and degradation of synaptic proteins.

Mutations in TRIM32 cause Limb-Girdle Muscular Dystrophy type 2H and Bardet-Biedl syndrome, disorders characterized by muscle weakness, retinal degeneration, and developmental defects. These mutations often impair E3 ligase activity or disrupt substrate binding, leading to accumulation of misfolded or damaged proteins. TRIM32 is also implicated in tumorigenesis, acting as either a tumor suppressor or oncogene depending on cellular context. Its ubiquitination of MYC and p53 pathway components links it directly to cancer cell proliferation and apoptosis.

Because TRIM32 integrates ubiquitin signaling with cellular differentiation and homeostasis, it serves as an essential node in protein quality control and tissue development. NSJ Bioreagents provides a validated TRIM32 antibody optimized for western blot, immunofluorescence, and ubiquitination assays, supporting research into muscle physiology, neurodegeneration, and cancer signaling.

UniProt: Q13049

Pathways: Negative Regulation of intrinsic apoptotic Signaling