MCMBP anticorps (AA 234-534)

N° du produit ABIN7872605

Cet anticorps Lapin Polyclonal détecte spécifiquement MCMBP dans WB, ELISA et IP. Il présente une réactivité avec des échantillons de Humain, Rat et Souris.

Aperçu rapide pour MCMBP anticorps (AA 234-534) (ABIN7872605)

Antigène: MCMBP (Minichromosome Maintenance Complex Binding Protein (MCMBP))

Reactivité: Humain, Rat, Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp MCMBP est non-conjugé

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP)

Détail du produit anti-MCMBP anticorps

Épitope: AA 234-534

Fonction: MCMBP Antibody / MCM-binding protein

Purification: Immunogen affinity purified

Immunogène: E.coli-derived human MCMBP recombinant protein (Position: E234-E534) was used as the immunogen for the MCMBP antibody.

Isotype: IgG

Information d'application

Indications d'application: Optimal dilution of the MCMBP antibody should be determined by the researcher.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Stock: 4 °C,-20 °C

Stockage commentaire: After reconstitution, the MCMBP antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Détails sur MCMBP

Antigène: MCMBP (Minichromosome Maintenance Complex Binding Protein (MCMBP))

Autre désignation: MCMBP

Sujet: MCMBP antibody detects Minichromosome maintenance complex-binding protein, a nuclear protein that regulates DNA replication and cell cycle progression. MCMBP interacts with the MCM (minichromosome maintenance) helicase complex, ensuring its proper assembly and nuclear localization. It functions as a chaperone stabilizing MCM subunits before DNA replication initiation. The MCMBP antibody is valuable in studies of DNA replication licensing, genome stability, and cell proliferation control.

MCMBP is encoded by the MCMBP gene located on human chromosome 10q24.2. The protein is approximately 70 kilodaltons in size and associates with MCM subunits MCM2-MCM7. These interactions facilitate the transport of MCM complexes into the nucleus and their incorporation into pre-replication complexes at replication origins. MCMBP assists in maintaining replication fork integrity and prevents unscheduled replication events that could lead to genomic instability. It also contributes to the disassembly of replication machinery during mitotic exit.

The MCMBP antibody identifies the 70 kilodalton protein by western blot and reveals nuclear localization during S phase, with partial cytoplasmic presence in early G1. Immunofluorescence shows co-localization with replication foci, supporting its role in DNA synthesis regulation. Functional studies demonstrate that depletion of MCMBP causes replication stress, reduced origin firing, and accumulation of DNA damage markers such as gamma-H2AX. MCMBP interacts with replication factor C and proliferating cell nuclear antigen, integrating replication initiation with elongation control.

Beyond DNA replication, MCMBP is involved in maintaining chromosomal stability and has been linked to mitotic checkpoint regulation. Overexpression has been reported in certain cancers, including hepatocellular and colorectal carcinomas, where it may promote cell cycle progression. Conversely, its loss sensitizes cells to replication inhibitors, suggesting potential as a therapeutic target. NSJ Bioreagents provides a validated MCMBP antibody optimized for western blot and immunoprecipitation, supporting detailed exploration of replication licensing, genome integrity, and cell cycle dynamics.

UniProt: Q9BTE3

Pathways: Chromatin Binding