TTLL1 anticorps (AA 26-402)

N° du produit ABIN7873002

L’anticorps anti-TTLL1 Polyclonal Lapin est utilisé pour la détection de TTLL1 dans des échantillons de Humain, Souris et Rat. Il a été validé pour ELISA, IHC et WB.

Aperçu rapide pour TTLL1 anticorps (AA 26-402) (ABIN7873002)

Antigène: TTLL1 (Tubulin tyrosine Ligase-Like Family, Member 1 (TTLL1))

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp TTLL1 est non-conjugé

Application: ELISA, Immunohistochemistry (IHC), Western Blotting (WB)

Détail du produit anti-TTLL1 anticorps

Épitope: AA 26-402

Fonction: TTLL1 Antibody / Tubulin-tyrosine ligase-like protein 1

Purification: Immunogen affinity purified

Immunogène: E.coli-derived human TTLL1 recombinant protein (Position: V26-Q402) was used as the immunogen for the TTLL1 antibody.

Isotype: IgG

Information d'application

Indications d'application: Optimal dilution of the TTLL1 antibody should be determined by the researcher.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Stock: 4 °C,-20 °C

Stockage commentaire: After reconstitution, the TTLL1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Détails sur TTLL1

Antigène: TTLL1 (Tubulin tyrosine Ligase-Like Family, Member 1 (TTLL1))

Autre désignation: TTLL1

Sujet: The TTLL1 antibody targets Tubulin-tyrosine ligase-like protein 1, an enzyme encoded by the TTLL1 gene that catalyzes the polyglutamylation of tubulin. Tubulin-tyrosine ligase-like protein 1 modifies the C-terminal tails of alpha-tubulin, generating polyglutamate side chains that regulate microtubule stability, interactions with motor proteins, and axonal transport. The TTLL1 antibody enables researchers to study post-translational modification of microtubules and the functional specialization of the cytoskeleton in neuronal and ciliary systems.

Tubulin-tyrosine ligase-like protein 1 belongs to the tubulin tyrosine ligase-like family, a group of enzymes that mediate tubulin polyglutamylation and polyglycylation. It primarily acts on alpha-tubulin within stable microtubule arrays such as those found in neurons and cilia. The TTLL1 antibody supports visualization of enzyme expression in neuronal soma and axons, reflecting its enrichment in brain tissue and importance for neural function. Loss of TTLL1 leads to reduced tubulin polyglutamylation, defective neuronal transport, and abnormal ciliary movement.

Polyglutamylation modulates the binding of microtubule-associated proteins (MAPs) and molecular motors including kinesin and dynein. The TTLL1 antibody provides a valuable reagent for analyzing this regulatory layer of cytoskeletal control. Dysregulation of TTLL1 activity contributes to neurodegenerative and ciliopathy phenotypes due to altered microtubule organization. TTLL1-dependent modification also influences mitotic spindle assembly and chromosome segregation.

Tubulin-tyrosine ligase-like protein 1 localizes to the cytoplasm and is often concentrated at centrosomes and ciliary bases. The TTLL1 antibody supports localization studies revealing its participation in microtubule-anchoring regions. Mutations in TTLL1 have been associated with human developmental disorders involving cerebellar atrophy and cognitive delay, reinforcing its essential role in neuronal microtubule regulation.

The TTLL1 antibody performs effectively in western blotting, immunofluorescence, and immunohistochemistry, yielding distinct cytoplasmic and centrosomal staining. NSJ Bioreagents provides this antibody as a validated, high-specificity reagent for cytoskeleton, neurobiology, and cilia research. By enabling precise detection of Tubulin-tyrosine ligase-like protein 1, the TTLL1 antibody supports studies of microtubule modification, axonal transport, and cellular architecture maintenance.

UniProt: O95922