RanBP3 anticorps (AA 26-517)

N° du produit ABIN7873009

Cet anticorps anti-RanBP3 Polyclonal Lapin (ABIN7873009) détecte spécifiquement RanBP3 dans WB, ELISA, IHC, IF, ICC et FACS. L’anticorps est réactif avec des échantillons de Humain, Souris et Rat.

Aperçu rapide pour RanBP3 anticorps (AA 26-517) (ABIN7873009)

Antigène: RanBP3 (RANBP3) (RAN Binding Protein 3 (RANBP3))

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp RanBP3 est non-conjugé

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Détail du produit anti-RanBP3 anticorps

Épitope: AA 26-517

Fonction: RANBP3 Antibody / Ran-binding protein 3

Purification: Immunogen affinity purified

Immunogène: E.coli-derived human RANBP3 recombinant protein (Position: Q26-Q517) was used as the immunogen for the RANBP3 antibody.

Isotype: IgG

Information d'application

Indications d'application: Optimal dilution of the RANBP3 antibody should be determined by the researcher.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Stock: 4 °C,-20 °C

Stockage commentaire: After reconstitution, the RANBP3 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Détails sur RanBP3

Antigène: RanBP3 (RANBP3) (RAN Binding Protein 3 (RANBP3))

Autre désignation: RANBP3

Sujet: RANBP3 antibody detects Ran-binding protein 3, a nuclear transport factor that regulates nucleocytoplasmic trafficking and RNA export. The UniProt recommended name is Ran-binding protein 3 (RANBP3). This protein functions as a cofactor in the Ran GTPase cycle, controlling the directionality of transport across the nuclear pore complex (NPC). RANBP3 is a crucial mediator in the export of proteins and RNAs from the nucleus to the cytoplasm, contributing to overall genome expression and cellular homeostasis.

Functionally, RANBP3 antibody identifies a 607-amino-acid cytoplasmic and nuclear protein that interacts with Ran GTPase, CRM1 (also known as Exportin 1), and other nucleocytoplasmic transport components. RANBP3 facilitates CRM1-dependent export of cargo molecules by stabilizing the export complex in a RanGTP-bound state. It also acts as a cofactor promoting efficient release of exported cargo upon GTP hydrolysis. Through these functions, RANBP3 ensures proper distribution of transcription factors, mRNA, and ribonucleoprotein complexes between cellular compartments.

The RANBP3 gene is located on chromosome 19p13.3 and is ubiquitously expressed across mammalian tissues. It contains an N-terminal nuclear export signal, a Ran-binding domain, and multiple leucine-rich motifs responsible for cargo recognition. RANBP3 participates in nuclear export of Smad proteins, linking it directly to TGF-beta signaling. By facilitating Smad nuclear export, it modulates transcriptional responses to growth factors and cytokines. Its activity is regulated by phosphorylation and nuclear-cytoplasmic shuttling that respond to cell cycle and signaling cues.

In addition to its export functions, RANBP3 maintains genome integrity by controlling the localization of DNA repair factors and replication regulators. It also contributes to ribosome biogenesis and mRNA surveillance by interacting with nuclear export adaptors. Dysregulation of RANBP3 has been associated with tumor progression and antiviral defense, as viruses often hijack CRM1-dependent pathways to export viral RNA and proteins.

RANBP3 antibody is widely used in studies of nucleocytoplasmic transport, gene regulation, and signal transduction. It is suitable for immunoblotting, immunofluorescence, and nuclear fractionation assays to examine RANBP3 distribution and function. This antibody supports research into RNA export, TGF-beta signaling, and nuclear pore dynamics. In disease studies, RANBP3 detection helps elucidate altered nuclear transport mechanisms contributing to cancer and viral infection.

Structurally, RANBP3 contains a pleckstrin homology-like domain and a Ran-binding domain that coordinate GTPase activity and CRM1 interaction. Post-translational modifications such as phosphorylation at specific serine residues regulate its subcellular localization and export efficiency. NSJ Bioreagents provides RANBP3 antibody reagents validated for use in nucleocytoplasmic transport, transcriptional regulation, and signal transduction research.

UniProt: Q9H6Z4