Hexosaminidase A anticorps (AA 28-528)

N° du produit ABIN7873273

Cet anticorps anti-Hexosaminidase A Polyclonal Lapin (ABIN7873273) détecte spécifiquement Hexosaminidase A dans WB et ELISA. L’anticorps est réactif avec des échantillons de Rat.

Aperçu rapide pour Hexosaminidase A anticorps (AA 28-528) (ABIN7873273)

Antigène: Hexosaminidase A (HEXA)

Reactivité: Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Hexosaminidase A est non-conjugé

Application: Western Blotting (WB), ELISA

Détail du produit anti-Hexosaminidase A anticorps

Épitope: AA 28-528

Fonction: Hexosaminidase A Antibody / HEXA

Purification: Affinity purified

Immunogène: Recombinant rat protein (amino acids Q28-T528) was used as the immunogen for the Hexosaminidase A antibody.

Isotype: IgG

Information d'application

Indications d'application: Optimal dilution of the Hexosaminidase A antibody should be determined by the researcher.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Buffer: 0.5 mg/mL if reconstituted with 0.2 mL sterile DI water

Stock: 4 °C,-20 °C

Stockage commentaire: After reconstitution, the Hexosaminidase A antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Détails sur Hexosaminidase A

Antigène: Hexosaminidase A (HEXA)

Autre désignation: Hexosaminidase A

Sujet: HEXA, hexosaminidase A (alpha polypeptide), is an enzyme that in humans is encoded by the HEXA gene. Hexosaminidase A and the cofactor GM2 activator protein catalyze the degradation of the GM2 gangliosides and other molecules containing terminal N-acetyl hexosamines. The HEXA gene encodes the alpha subunit of hexosaminidase A , a lysosomal enzyme involved in the breakdown of gangliosides. The HEXA gene is mapped on 15q23. Even though the alpha and beta subunits of hexosaminidase A can both cleave GalNAc residues, only the alpha subunit is able to hydrolyze GM2 gangliosides. The alpha subunit contains a key residue, Arg-424, which is essential for binding the N-acetyl-neuramanic residue of GM2 gangliosides. Chimeric constructs were expressed in HeLa cells and selected constructs were produced in the baculovirus expression system to determine their ability to degrade GM2 ganglioside in the presence of GM2 activator protein. Their results allowed them to define 2 noncontiguous sequences in the alpha subunit (amino acids 1-191 and 403-529) which, when substituted into analogous positions in the beta subunit, conferred activity against the sulfated substrate.

UniProt: Q641X3

Pathways: Sensory Perception of Sound, Glycosaminoglycan Metabolic Process