Senataxin (SETX) (AA 29-2544) anticorps

N° du produit ABIN7873404

L’anticorps anti-Senataxin Polyclonal Lapin est utilisé pour la détection de Senataxin dans des échantillons de Humain. Il a été validé pour WB, ELISA et FACS.

Aperçu rapide pour Senataxin (SETX) (AA 29-2544) anticorps (ABIN7873404)

Antigène: Senataxin (SETX)

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Inconjugué

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Détail du produit

Épitope: AA 29-2544

Fonction: Senataxin Antibody / SETX

Purification: Immunogen affinity purified

Immunogène: E.coli-derived human SETX recombinant protein (Position: E29-R2544) was used as the immunogen for the Senataxin antibody.

Isotype: IgG

Information d'application

Indications d'application: Optimal dilution of the Senataxin antibody should be determined by the researcher.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Stock: 4 °C,-20 °C

Stockage commentaire: After reconstitution, the Senataxin antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Détail du antigène

Antigène: Senataxin (SETX)

Autre désignation: Senataxin

Sujet: Senataxin antibody detects DNA/RNA helicase Senataxin, encoded by the SETX gene located on chromosome 9q34.13. Senataxin is a large nuclear helicase that unwinds DNA/RNA hybrids known as R-loops, thereby maintaining genomic stability during transcription and replication. It plays crucial roles in transcription termination, RNA processing, and DNA damage repair, linking transcriptional control with genome maintenance. Senataxin is expressed widely, with elevated levels in neurons, germ cells, and proliferative tissues where transcription-coupled DNA repair is highly active.

Structurally, Senataxin is a 2677-amino-acid helicase belonging to the superfamily 1 (SF1) of DNA/RNA helicases. It contains a C-terminal helicase domain with conserved Walker A and B motifs for ATP binding and hydrolysis, an N-terminal region that mediates protein-protein interactions, and nuclear localization sequences. The helicase activity of Senataxin resolves R-loop structures formed during transcription, preventing collisions between transcription and replication machinery. Co-localization studies show Senataxin enriched in nuclear foci associated with sites of active transcription and DNA repair.

Functionally, Senataxin maintains genome integrity by resolving R-loops and facilitating proper termination of RNA polymerase II transcription. It contributes to DNA double-strand break repair via homologous recombination and regulates transcription-coupled nucleotide excision repair. Senataxin also modulates RNA maturation by interacting with RNA processing factors such as XRN2 and the exosome complex. In neurons, it supports axonal maintenance and stress granule dynamics, while in germ cells, it regulates meiotic recombination and spermatogenesis.

Mutations in the SETX gene are linked to several neurodegenerative and neuromuscular disorders, including ataxia with oculomotor apraxia type 2 (AOA2) and juvenile amyotrophic lateral sclerosis type 4 (ALS4). These conditions are characterized by progressive neurodegeneration and impaired DNA repair capacity. Senataxin deficiency leads to accumulation of R-loops, transcriptional stress, and genomic instability. Pathway associations include RNA processing, DNA damage response, and transcription regulation. During development, Senataxin expression supports neuronal differentiation and genome maintenance in post-mitotic cells.

The Senataxin antibody from NSJ Bioreagents is a valuable reagent for studying transcription-coupled repair, RNA metabolism, and neurodegenerative disease mechanisms.

UniProt: Q7Z333