GLRX3 anticorps (AA 31-307)

N° du produit ABIN7873780

Cet anticorps anti-GLRX3 Polyclonal Lapin (ABIN7873780) détecte spécifiquement GLRX3 dans WB, ELISA et FACS. L’anticorps est réactif avec des échantillons de Humain, Souris et Rat.

Aperçu rapide pour GLRX3 anticorps (AA 31-307) (ABIN7873780)

Antigène: GLRX3 (Glutaredoxin 3 (GLRX3))

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp GLRX3 est non-conjugé

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Détail du produit anti-GLRX3 anticorps

Épitope: AA 31-307

Fonction: GLRX3 Antibody / Glutaredoxin 3 / PICOT

Purification: Immunogen affinity purified

Immunogène: E.coli-derived human GLRX3 recombinant protein (Position: K31-V307) was used as the immunogen for the GLRX3 antibody.

Isotype: IgG

Information d'application

Indications d'application: Optimal dilution of the GLRX3 antibody should be determined by the researcher.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Stock: 4 °C,-20 °C

Stockage commentaire: After reconstitution, the GLRX3 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Détails sur GLRX3

Antigène: GLRX3 (Glutaredoxin 3 (GLRX3))

Autre désignation: GLRX3

Sujet: GLRX3 antibody detects Glutaredoxin-3 (also known as PICOT or Thioredoxin-like protein 2), a multifunctional redox enzyme that regulates cellular redox homeostasis, iron-sulfur cluster assembly, and signal transduction. Encoded by the GLRX3 gene on chromosome 10q26.3, this protein belongs to the glutaredoxin family, characterized by their thiol-disulfide oxidoreductase activity. GLRX3 contains two monothiol glutaredoxin domains that utilize glutathione to catalyze reversible thiol exchange reactions, thereby maintaining protein thiol status and redox balance. Beyond redox control, GLRX3 acts as a scaffold protein for Fe-S cluster delivery and as a modulator of intracellular signaling cascades.

GLRX3 is ubiquitously expressed but particularly abundant in heart, skeletal muscle, and liver, where it contributes to mitochondrial function and oxidative stress resistance. The protein forms complexes with BolA-like proteins to facilitate Fe-S cluster biogenesis, which is essential for electron transport chain integrity. In cardiomyocytes, GLRX3i 1/2originally identified as PICOT (PKC-interacting cousin of thioredoxin)i 1/2binds to protein kinase C theta and inhibits its activity, modulating hypertrophic signaling and contractility. This redox-regulatory interaction links GLRX3 to cardiac protection against oxidative damage and maladaptive stress responses.

The GLRX3 antibody is widely used in redox biology, metabolism, and cardiovascular research to examine oxidative stress regulation, Fe-S cluster metabolism, and cell signaling. Western blot analysis identifies a 38 kilodalton band corresponding to GLRX3, while immunofluorescence shows cytoplasmic and mitochondrial localization. Studies using the antibody have revealed that GLRX3 depletion leads to increased ROS levels, impaired mitochondrial respiration, and reduced iron homeostasis. Because these effects contribute to cellular senescence and disease progression, GLRX3 is of growing interest as a therapeutic target in oxidative and metabolic disorders.

Beyond its roles in redox and energy metabolism, GLRX3 contributes to developmental processes and immune function. It modulates NF-?B and MAPK pathways, influencing inflammatory gene expression. Overexpression has been reported in cancers such as hepatocellular carcinoma and breast carcinoma, where it promotes proliferation and resistance to oxidative stress. The GLRX3 antibody therefore provides a valuable reagent for investigating molecular mechanisms of redox regulation, signal transduction, and disease adaptation. NSJ Bioreagents supplies this antibody validated for its applications, ensuring high specificity and reproducibility for both basic and translational research applications.

UniProt: O76003

Pathways: Cell RedoxHomeostasis