UBR5 anticorps (AA 31-761)

N° du produit ABIN7873803

L’anticorps anti-UBR5 Polyclonal Lapin est utilisé pour la détection de UBR5 dans des échantillons de Humain, Souris et Rat. Il a été validé pour WB, ELISA et FACS.

Aperçu rapide pour UBR5 anticorps (AA 31-761) (ABIN7873803)

Antigène: UBR5 (Ubiquitin Protein Ligase E3 Component N-Recognin 5 (UBR5))

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp UBR5 est non-conjugé

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Détail du produit anti-UBR5 anticorps

Épitope: AA 31-761

Fonction: UBR5 Antibody / hHYD

Purification: Immunogen affinity purified

Immunogène: E.coli-derived human UBR5 recombinant protein (Position: K31-K761) was used as the immunogen for the UBR5 antibody.

Isotype: IgG

Information d'application

Indications d'application: Optimal dilution of the UBR5 antibody should be determined by the researcher.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Stock: 4 °C,-20 °C

Stockage commentaire: After reconstitution, the UBR5 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Détails sur UBR5

Antigène: UBR5 (Ubiquitin Protein Ligase E3 Component N-Recognin 5 (UBR5))

Autre désignation: UBR5

Sujet: UBR5 antibody detects E3 ubiquitin-protein ligase UBR5, a large multidomain enzyme that catalyzes ubiquitination of specific substrate proteins, regulating DNA damage repair, transcription, and cell cycle control. UBR5 belongs to the HECT-type E3 ligase family and plays a critical role in maintaining genomic integrity and proteostasis. The UBR5 antibody is widely used in studies of ubiquitin signaling, cancer biology, and DNA repair mechanisms.

UBR5 is encoded by the UBR5 gene located on human chromosome 8q22.3. The protein is approximately 300 kilodaltons and contains a C-terminal HECT domain responsible for ubiquitin transfer, an N-terminal UBR box involved in substrate recognition, and several WW domains that mediate protein-protein interactions. UBR5 localizes to both the nucleus and cytoplasm, functioning in proteasomal degradation and signaling complex assembly.

The UBR5 antibody detects a 300 kilodalton band in western blot analysis and shows both nuclear and cytoplasmic staining under immunofluorescence. UBR5 ubiquitinates regulators of the DNA damage response such as BRCA1 and p53, coordinating checkpoint activation and repair. It also targets transcription factors and signaling intermediates, integrating stress responses with transcriptional control.

UBR5 is essential for embryonic development and cell viability. Its dysregulation is associated with multiple cancers, including breast, ovarian, and colorectal carcinoma, where it functions as either an oncogene or tumor suppressor depending on context. Overexpression enhances proliferation and metastasis, while depletion sensitizes cells to DNA-damaging agents and promotes apoptosis.

As a master regulator of ubiquitin signaling, UBR5 links proteolytic control with cell fate decisions. NSJ Bioreagents provides a validated UBR5 antibody optimized for western blot and flow cytometry, supporting research into ubiquitination pathways, DNA damage responses, and oncogenic signaling networks.

UniProt: O95071

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway