INPP5F anticorps (AA 317-1112)

N° du produit ABIN7873893

L’anticorps anti-INPP5F Polyclonal Lapin est utilisé pour la détection de INPP5F dans des échantillons de Humain. Il a été validé pour WB, ELISA et FACS.

Aperçu rapide pour INPP5F anticorps (AA 317-1112) (ABIN7873893)

Antigène: INPP5F (Inositol Polyphosphate-5-Phosphatase F (INPP5F))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp INPP5F est non-conjugé

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Détail du produit anti-INPP5F anticorps

Épitope: AA 317-1112

Fonction: INPP5F Antibody / Type II inositol 1,4,5-trisphosphate 5-phosphatase

Purification: Immunogen affinity purified

Immunogène: E.coli-derived human INPP5F recombinant protein (Position: H317-Q1112) was used as the immunogen for the INPP5F antibody.

Isotype: IgG

Information d'application

Indications d'application: Optimal dilution of the INPP5F antibody should be determined by the researcher.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Stock: 4 °C,-20 °C

Stockage commentaire: After reconstitution, the INPP5F antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Détails sur INPP5F

Antigène: INPP5F (Inositol Polyphosphate-5-Phosphatase F (INPP5F))

Autre désignation: INPP5F

Sujet: INPP5F antibody detects Type II inositol 1,4,5-trisphosphate 5-phosphatase (also known as phosphatidylinositol polyphosphate 5-phosphatase INPP5F), an enzyme that regulates phosphoinositide metabolism and intracellular signaling. Encoded by the INPP5F gene on chromosome 10q26.11, this protein hydrolyzes the 5-phosphate group from phosphatidylinositol (4,5)-bisphosphate and phosphatidylinositol (3,4,5)-trisphosphate, thereby modulating PI3K/AKT and phosphatidylinositol signaling pathways. By controlling local phosphoinositide concentrations, INPP5F regulates membrane dynamics, vesicle trafficking, and cardiac hypertrophy signaling. It acts as a negative regulator of PI3K-dependent cell growth and survival, contributing to cellular homeostasis and organ size control.

INPP5F localizes to the cytoplasm and Golgi apparatus and is expressed in multiple tissues, with highest levels in heart, brain, and skeletal muscle. Functional studies have demonstrated that INPP5F attenuates insulin and growth factor signaling by dephosphorylating PIP3, thereby reducing AKT activation. In cardiomyocytes, INPP5F serves as an intrinsic brake on hypertrophic signaling by modulating the PI3K/AKT/mTOR axis. Mice lacking INPP5F display enhanced cardiac growth and increased susceptibility to heart failure, underscoring its physiological importance in maintaining normal cardiac morphology and function.

The INPP5F antibody is widely used in signal transduction, cardiovascular, and metabolic research to study phosphoinositide regulation and AKT signaling. Western blot analysis typically reveals a 108 kilodalton band corresponding to the full-length enzyme. Immunofluorescence shows perinuclear and Golgi-associated staining patterns consistent with its subcellular localization. Because INPP5F interacts with lipid-binding proteins and signaling kinases, this antibody is also employed in co-immunoprecipitation and pathway-mapping assays. Reduced INPP5F expression has been observed in glioblastoma, prostate cancer, and other tumors, suggesting its function as a tumor suppressor through attenuation of PI3K-driven oncogenic pathways.

In addition to its phosphatase activity, INPP5F contributes to endosomal sorting and receptor recycling by shaping local phosphoinositide pools on endomembranes. It cooperates with SHIP2 and synaptojanin to balance phosphoinositide turnover in response to receptor activation. The INPP5F antibody enables detailed examination of these signaling processes and provides insight into how lipid signaling defects contribute to disease. NSJ Bioreagents supplies this antibody validated for its applications, ensuring reliable detection in cellular and tissue models of phosphoinositide regulation.

UniProt: Q9Y2H2