DDX39B anticorps (AA 351-380)

N° du produit ABIN7874225

Cet anticorps Lapin Polyclonal détecte spécifiquement DDX39B dans WB, FACS et IHC (p). Il présente une réactivité avec des échantillons de Humain.

Aperçu rapide pour DDX39B anticorps (AA 351-380) (ABIN7874225)

Antigène: DDX39B (DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 39B (DDX39B))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp DDX39B est non-conjugé

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Détail du produit anti-DDX39B anticorps

Épitope: AA 351-380

Fonction: DDX39B Antibody / UAP56

Purification: Antigen affinity purified

Immunogène: A portion of amino acids 351-380 from the human protein was used as the immunogen for the DDX39B antibody.

Isotype: Ig Fraction

Information d'application

Indications d'application: The stated application concentrations are suggested starting points. Titration of the DDX39B antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: In 1X PBS, pH 7.4, with 0.09 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Aliquot the DDX39B antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Détails sur DDX39B

Antigène: DDX39B (DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 39B (DDX39B))

Autre désignation: DDX39B

Sujet: Component of the THO subcomplex of the TREX complex. The TREX complex specifically associates with spliced mRNA and not with unspliced pre-mRNA. It is recruited to spliced mRNAs by a transcription-independent mechanism. Binds to mRNA upstream of the exon-junction complex (EJC) and is recruited in a splicing-and cap-dependent manner to a region near the 5' end of the mRNA where it functions in mRNA export. The recruitment occurs via an interaction between THOC4 and the cap-binding protein NCBP1. UAP56 functions as a bridge between THOC4 and the THO complex. The TREX complex is essential for the export of Kaposi's sarcoma-associated herpesvirus (KSHV) intronless mRNAs and infectious virus production. The recruitment of the TREX complex to the intronless viral mRNA occurs via an interaction between KSHV ORF57 protein and THOC4. Splice factor that is required for the first ATP-dependent step in spliceosome assembly and for the interaction of U2 snRNP with the branchpoint. It has both RNA-stimulated ATP binding/hydrolysis activity and ATP-dependent RNA unwinding activity. Even with the stimulation of RNA, the ATPase activity is weak. It can only hydrolyze ATP but not other NTPs. The RNA stimulation of ATPase activity does not have a strong preference for the sequence and length of the RNA. However, ssRNA stimulates the ATPase activity much more strongly than dsRNA. It can unwind 5' or 3' overhangs or blunt end RNA duplexes in vitro. The ATPase and helicase activities are not influenced by U2AF2 and THOC4.

UniProt: Q13838

Pathways: Ribonucleoprotein Complex Subunit Organization