PIK3R4 anticorps (AA 36-1339)

N° du produit ABIN7874291

Cet anticorps Lapin Polyclonal détecte spécifiquement PIK3R4 dans ELISA, WB et FACS. Il présente une réactivité avec des échantillons de Humain.

Aperçu rapide pour PIK3R4 anticorps (AA 36-1339) (ABIN7874291)

Antigène: PIK3R4 (Phosphoinositide 3-kinase Regulatory Subunit 4 (PIK3R4))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp PIK3R4 est non-conjugé

Application: ELISA, Western Blotting (WB), Flow Cytometry (FACS)

Détail du produit anti-PIK3R4 anticorps

Épitope: AA 36-1339

Fonction: PIK3R4 Antibody / Phosphoinositide 3-kinase regulatory subunit 4

Purification: Antigen affinity chromatography

Immunogène: An E.coli-derived human recombinant protein (amino acids R36-Q1339) was used as the immunogen for the PIK3R4 antibody.

Isotype: IgG

Information d'application

Indications d'application: Optimal dilution of the PIK3R4 antibody should be determined by the researcher.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Buffer: 0.5 mg/mL if reconstituted with 0.2 mL sterile DI water

Stock: 4 °C,-20 °C

Stockage commentaire: After reconstitution, the PIK3R4 Antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Détails sur PIK3R4

Antigène: PIK3R4 (Phosphoinositide 3-kinase Regulatory Subunit 4 (PIK3R4))

Autre désignation: PIK3R4

Sujet: Phosphoinositide 3-kinase regulatory subunit 4, also known as PI3-kinase regulatory subunit 4 or PI3-kinase p150 subunit or phosphoinositide 3-kinase adaptor protein, or VPS15 is an enzyme that in humans is encoded by the PIK3R4 gene. The serine/threonine PI3 kinase regulatory subunit 4 (PIK3R4, Vps15) is the mammalian homologue of the yeast vacuolar protein sorting 15. PIK3R4 regulates the kinase activity of PI3K class III and anchors the kinase to cellular membranes through myristoylation. Recruitment of PI3K class III to the site of early endosome fusion and docking is directly mediated by PIK3R4 binding to the small GTPase Rab5 through its HEAT and WD-40 domains. The PIK3R4/PI3K class III plays a role in late endosome function through PIK3R4 binding to the Rab7 GTPase. In addition to its role in trafficking, the PIK3R4/PI3K class III complex interacts with beclin-1 to play a role during several stages of autophagy. Autophagosome formation is stimulated when Atg14 complexes with PIK3R4, PI3K class III, and beclin-1. The UVRAG protein competes with Atg14 for beclin-1 binding, forming a mutually exclusive complex with PIK3R4, PI3K class III, and beclin-1 that regulates autophagosome maturation. Autophagosome maturation is impaired in the presence of the beclin-1-binding protein Rubicon. Co-expression of PIK3R4 is required for PI3K class III activation and regulation by both beclin-1/UVRAG and by nutrients. Overexpression of PIK3R4 protein has been associated with decreased survival in patients with ovarian tumors, while mutations of the corresponding PIK3R4 gene are associated with metastatic melanoma, suggesting that PIK3R4 functions in cancer.

UniProt: Q99570