RLIM anticorps (AA 369-621)

N° du produit ABIN7874379

L’anticorps anti-RLIM Polyclonal Lapin est utilisé pour la détection de RLIM dans des échantillons de Humain, Souris et Rat. Il a été validé pour WB, ELISA, IHC, IF et FACS.

Aperçu rapide pour RLIM anticorps (AA 369-621) (ABIN7874379)

Antigène: RLIM (Ring Finger Protein, LIM Domain Interacting (RLIM))

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp RLIM est non-conjugé

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Détail du produit anti-RLIM anticorps

Épitope: AA 369-621

Fonction: RLIM Antibody / RING finger protein LIM domain-interacting

Purification: Immunogen affinity purified

Immunogène: E.coli-derived human RLIM recombinant protein (Position: R369-E621) was used as the immunogen for the RLIM antibody.

Isotype: IgG

Information d'application

Indications d'application: Optimal dilution of the RLIM antibody should be determined by the researcher.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Stock: 4 °C,-20 °C

Stockage commentaire: After reconstitution, the RLIM antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Détails sur RLIM

Antigène: RLIM (Ring Finger Protein, LIM Domain Interacting (RLIM))

Autre désignation: RLIM

Sujet: RLIM antibody detects RING finger protein LIM domain-interacting (RLIM), also known as RNF12, encoded by the RLIM gene on chromosome Xq13.2. RLIM antibody is commonly used in studies of ubiquitination, X-chromosome inactivation, and transcriptional regulation. RLIM is an E3 ubiquitin ligase that regulates protein stability, signaling, and developmental processes. It is expressed broadly, with high levels in embryonic tissues and reproductive organs, and is essential for early embryogenesis and sex-specific gene regulation.

Structurally, RLIM is a ~600 amino acid protein containing a C-terminal RING finger domain responsible for ubiquitin ligase activity and N-terminal regions that mediate protein-protein interactions. It interacts with LIM domain transcription factors, modulating their activity and stability. Alternative splicing generates multiple isoforms with differences in subcellular localization and activity. Nuclear and cytoplasmic pools of RLIM allow it to function in both transcriptional regulation and cytoplasmic protein turnover.

Functionally, RLIM acts as an E3 ubiquitin ligase, tagging proteins for proteasomal degradation. It regulates LIM homeodomain transcription factors by modulating their stability, thereby controlling gene expression programs during development. RLIM also plays a crucial role in X-chromosome inactivation by promoting degradation of the transcription factor REX1, enabling proper expression of Xist RNA. Additional roles include regulation of BMP and TGF-b signaling pathways, neuronal differentiation, and germ cell development. Researchers use RLIM antibody to study ubiquitination, transcriptional regulation, and embryonic development.

Clinically, RLIM mutations are associated with intellectual disability, developmental delay, and congenital anomalies, reflecting its importance in brain and body development. As an X-linked gene, mutations often present with sex-specific patterns of inheritance. RLIM dysfunction has been implicated in cancer, where altered ubiquitination affects cell growth and signaling. NSJ Bioreagents provides RLIM antibody for developmental biology, cancer, and molecular signaling research.

Experimentally, RLIM antibody is used in western blotting to detect the ~68 kDa protein, in immunofluorescence to study nuclear and cytoplasmic localization, and in immunohistochemistry to examine expression in embryonic and tumor tissues. Co-immunoprecipitation with RLIM antibody identifies interacting transcription factors and ubiquitination substrates.

UniProt: Q9NVW2