Involucrin anticorps (AA 421-568)

N° du produit ABIN7874933

Cet anticorps Souris Monoclonal détecte spécifiquement Involucrin dans IHC (p). Il présente une réactivité avec des échantillons de Humain.

Aperçu rapide pour Involucrin anticorps (AA 421-568) (ABIN7874933)

Antigène: Involucrin (IVL)

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Involucrin est non-conjugé

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: SPM259

Détail du produit anti-Involucrin anticorps

Épitope: AA 421-568

Fonction: Involucrin Antibody

Purification: Protein G affinity chromatography

Immunogène: Purified involucrin from human keratinocytes was used as the immunogen for this Involucrin antibody. Its epitope maps between amino acids 421-568 of human involucrin.

Isotype: IgG1, kappa

Information d'application

Indications d'application: The optimal dilution of the Involucrin antibody for each application should be determined by the researcher.

1. Staining of formalin-fixed tissues is enhanced by digestion with Trypsin or Protease XXV at 1 mg/mL PBS for 5 min at 37oC. Enzyme digestion is better than citrate buffer based epitope unmasking.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: Prediluted in 1X PBS, 0.1 mg/mL BSA (US sourced), 0.05 % sodium azide, *For IHC use only*

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store the Involucrin antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Détails sur Involucrin

Antigène: Involucrin (IVL)

Autre désignation: Involucrin

Sujet: Involucrin is expressed in a range of stratified squamous epithelia, including the cornea, which lacks a distinct cornified layer. In normal epidermis, it is first expressed in the upper spinous layers, and in keratinocyte cultures, all cells that have left the basal layer express it. Involucrin expression is altered in pathological conditions: in psoriasis and other benign epidermal hyperplasias, involucrin expression begins closer to the basal layer than normal, expression is abnormal in squamous cell carcinomas and premalignant lesions, and is reduced in severe dysplasias of the larynx and cervix.

UniProt: P07476