NASP anticorps (AA 442-788)

N° du produit ABIN7875036

L’anticorps anti-NASP Polyclonal Lapin est utilisé pour la détection de NASP dans des échantillons de Humain, Rat et Souris. Il a été validé pour WB, IHC, ELISA, IF, FACS, IP et ICC.

Aperçu rapide pour NASP anticorps (AA 442-788) (ABIN7875036)

Antigène: NASP (Nuclear Autoantigenic Sperm Protein (Histone-Binding) (NASP))

Reactivité: Humain, Rat, Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp NASP est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Détail du produit anti-NASP anticorps

Épitope: AA 442-788

Fonction: NASP antibody / Nuclear autoantigenic sperm

Purification: Immunogen affinity purified

Immunogène: E.coli-derived human NASP recombinant protein (Position: K442-C788)

Isotype: IgG

Information d'application

Indications d'application: Optimal dilution of the NASP antibody should be determined by the researcher.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Stock: -20 °C

Stockage commentaire: Store the NASP antibody at -20oC.

Détails sur NASP

Antigène: NASP (Nuclear Autoantigenic Sperm Protein (Histone-Binding) (NASP))

Autre désignation: NASP

Sujet: NASP antibody detects Nuclear autoantigenic sperm protein, encoded by the NASP gene on chromosome 1q24.2. NASP antibody is widely used in studies of histone metabolism, chromatin biology, and fertility. NASP is a histone chaperone that binds histones H1 and H3/H4, facilitating their storage, transport, and deposition onto DNA during chromatin assembly. By regulating histone availability, NASP ensures proper nucleosome formation, DNA replication, and cell cycle progression.

Structurally, NASP is a ~85 kDa protein that exists in two major isoforms: somatic NASP and testis-specific NASP. Both isoforms contain tetratricopeptide repeat (TPR) domains that mediate histone binding and interactions with other chaperones. Testis-specific NASP is highly expressed in germ cells and plays essential roles in spermatogenesis. Somatic NASP is expressed broadly across proliferating cells, including embryonic and cancer cells.

Functionally, NASP delivers histones to chromatin assembly factors and prevents their aggregation or degradation. It partners with histone-binding proteins such as ASF1 and HSPA2 to facilitate nucleosome assembly. By regulating histone pools, NASP maintains genomic stability and supports DNA replication. Researchers use NASP antibody to investigate chromatin assembly, fertility, and cancer biology.

Clinically, NASP is implicated in infertility, particularly male infertility, due to its essential role in spermatogenesis. Dysregulation of NASP has also been reported in cancers, where altered histone chaperone function contributes to genomic instability and abnormal gene expression. Autoantibodies against NASP have been described in certain autoimmune conditions. NSJ Bioreagents supplies NASP antibody for research in fertility, chromatin regulation, and oncology.

Experimentally, NASP antibody is used in western blotting to detect both somatic and testis-specific isoforms, in immunohistochemistry to analyze germ cell and tumor tissue expression, and in co-immunoprecipitation to study histone-chaperone complexes.

UniProt: P49321