PARP15 anticorps (AA 47-555)

N° du produit ABIN7875163

Cet anticorps anti-PARP15 Polyclonal Lapin (ABIN7875163) détecte spécifiquement PARP15 dans WB, ELISA, FACS, ICC, IF et IHC. L’anticorps est réactif avec des échantillons de Humain, Souris et Rat.

Aperçu rapide pour PARP15 anticorps (AA 47-555) (ABIN7875163)

Antigène: PARP15 (Poly (ADP-Ribose) Polymerase Family, Member 15 (PARP15))

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp PARP15 est non-conjugé

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC)

Détail du produit anti-PARP15 anticorps

Épitope: AA 47-555

Fonction: PARP15 Antibody / Poly ADP-ribose polymerase 15

Purification: Immunogen affinity purified

Immunogène: E.coli-derived human PARP15 recombinant protein (Position: N47-R555) was used as the immunogen for the PARP15 antibody.

Isotype: IgG

Information d'application

Indications d'application: Optimal dilution of the PARP15 antibody should be determined by the researcher.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Stock: 4 °C,-20 °C

Stockage commentaire: After reconstitution, the PARP15 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Détails sur PARP15

Antigène: PARP15 (Poly (ADP-Ribose) Polymerase Family, Member 15 (PARP15))

Autre désignation: PARP15

Sujet: PARP15 antibody recognizes Poly (ADP-ribose) polymerase 15, an enzyme belonging to the PARP family involved in ADP-ribosylation, a post-translational modification regulating transcription, DNA repair, and stress responses. Unlike the classical DNA repair PARPs, PARP15 functions primarily in transcriptional repression and RNA processing. It possesses both mono-ADP-ribosyltransferase and macro domains, allowing it to modify specific target proteins. The PARP15 antibody is an essential reagent for studies of ADP-ribosylation signaling, chromatin dynamics, and cellular stress adaptation.

PARP15 is encoded by the PARP15 gene located on human chromosome 3q21.1. The protein comprises two N-terminal macro domains, a WWE domain involved in proteini 1/2protein interactions, and a C-terminal catalytic PARP domain. PARP15 localizes mainly to the nucleus and cytoplasmic granules, where it may regulate mRNA metabolism and response to stress. It has been implicated in cell survival pathways, as well as in the regulation of transcription factors under conditions of nutrient deprivation or oxidative stress.

Research using the PARP15 antibody has demonstrated its involvement in cancer cell proliferation and inflammation. Aberrant expression of PARP15 has been observed in lymphomas and certain solid tumors, where it may contribute to altered transcriptional control and resistance to apoptosis. Western blot analysis typically detects a band at approximately 74i 1/278 kDa. Immunocytochemical staining shows both nuclear and cytoplasmic localization, depending on cell type and stress conditions. Functional studies suggest that PARP15 can auto-modify and regulate its own enzymatic activity, similar to other PARP family members.

In recent years, PARP15 has attracted attention as part of the broader ADP-ribosylation signaling network, which includes enzymes, hydrolases, and binding proteins that control protein modification during stress responses. The antibody supports exploration of this enzyme's role in RNA granule formation, viral defense, and oncogenic signaling pathways. NSJ Bioreagents provides a validated PARP15 antibody for applications such as western blot, immunohistochemistry, and immunofluorescence, ensuring reliable results across molecular biology and cancer research contexts.

UniProt: Q460N3