FOXM1 anticorps (AA 48-763)

N° du produit ABIN7875216

L’anticorps anti-FOXM1 Polyclonal Lapin est utilisé pour la détection de FOXM1 dans des échantillons de Humain, Rat et Souris. Il a été validé pour WB, ELISA, ICC, IF et FACS.

Aperçu rapide pour FOXM1 anticorps (AA 48-763) (ABIN7875216)

Antigène: FOXM1 (Forkhead Box M1 (FOXM1))

Reactivité: Humain, Rat, Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp FOXM1 est non-conjugé

Application: Western Blotting (WB), ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FACS)

Détail du produit anti-FOXM1 anticorps

Épitope: AA 48-763

Fonction: FOXM1 Antibody / Forkhead box protein M1

Purification: Immunogen affinity purified

Immunogène: E.coli-derived human FOXM1 recombinant protein (Position: K48-Q763) was used as the immunogen for the FOXM1 antibody.

Isotype: IgG

Information d'application

Indications d'application: Optimal dilution of the FOXM1 antibody should be determined by the researcher.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Stock: 4 °C,-20 °C

Stockage commentaire: After reconstitution, the FOXM1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Détails sur FOXM1

Antigène: FOXM1 (Forkhead Box M1 (FOXM1))

Autre désignation: FOXM1

Sujet: FOXM1 antibody detects Forkhead box protein M1, encoded by the FOXM1 gene on chromosome 12p13.33. FOXM1 antibody is widely used in studies of cell cycle regulation, transcription, and cancer biology. FOXM1 is a member of the forkhead box (FOX) family of transcription factors, characterized by a conserved winged-helix DNA-binding domain. It plays critical roles in G1/S and G2/M transitions of the cell cycle, controlling genes required for DNA replication, mitosis, and cytokinesis. Expression is high in proliferating cells and embryonic tissues, but normally downregulated in differentiated, non-dividing cells.

Structurally, FOXM1 contains an N-terminal repressor domain, a central forkhead DNA-binding domain, and a C-terminal transactivation domain. Its activity is regulated by phosphorylation, with cyclin-CDK complexes and MAPKs activating FOXM1 during the cell cycle. Alternative splicing produces isoforms with distinct transcriptional activities, including FOXM1a (inactive), FOXM1b, and FOXM1c (active in proliferation).

Functionally, FOXM1 drives expression of cell cycle regulators such as cyclin B1, cyclin D1, PLK1, and Aurora B kinase. It ensures proper chromosome segregation, spindle assembly, and DNA repair. Loss of FOXM1 impairs proliferation and causes mitotic defects, while overexpression accelerates tumorigenesis. Researchers use FOXM1 antibody to study transcriptional regulation, cell cycle control, and tumor progression.

Clinically, FOXM1 is strongly associated with cancer. Overexpression occurs in breast, liver, prostate, lung, and brain tumors, correlating with aggressive phenotypes and poor prognosis. FOXM1 promotes tumor growth by enhancing proliferation, metastasis, angiogenesis, and therapy resistance. Targeting FOXM1 is an emerging therapeutic strategy, with inhibitors under development. Beyond oncology, FOXM1 also participates in tissue repair, stem cell biology, and aging processes. NSJ Bioreagents provides FOXM1 antibody for use in oncology, stem cell, and regenerative medicine research.

Experimentally, FOXM1 antibody is applied in western blotting to detect the ~80i 1/290 kDa protein, in immunohistochemistry to assess tumor expression, and in immunofluorescence microscopy to study nuclear localization. ChIP assays using FOXM1 antibody help identify direct gene targets of this transcription factor.

UniProt: Q08050

Pathways: Positive Regulation of Response to DNA Damage Stimulus