OTUD6B anticorps (AA 66-293)

N° du produit ABIN7876335

L’anticorps anti-OTUD6B Polyclonal Lapin est utilisé pour la détection de OTUD6B dans des échantillons de Humain. Il a été validé pour WB, ELISA et FACS.

Aperçu rapide pour OTUD6B anticorps (AA 66-293) (ABIN7876335)

Antigène: OTUD6B (OTU Domain Containing 6B (OTUD6B))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp OTUD6B est non-conjugé

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Détail du produit anti-OTUD6B anticorps

Épitope: AA 66-293

Fonction: OTUD6B Antibody / OTU domain-containing deubiquitinase 6B

Purification: Immunogen affinity purified

Immunogène: E.coli-derived human OTUD6B recombinant protein (Position: E66-S293) was used as the immunogen for the OTUD6B antibody.

Isotype: IgG

Information d'application

Indications d'application: Optimal dilution of the OTUD6B antibody should be determined by the researcher.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Stock: 4 °C,-20 °C

Stockage commentaire: After reconstitution, the OTUD6B antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Détails sur OTUD6B

Antigène: OTUD6B (OTU Domain Containing 6B (OTUD6B))

Autre désignation: OTUD6B

Sujet: OTUD6B antibody detects OTU domain-containing deubiquitinase 6B, a cysteine protease encoded by the OTUD6B gene located on chromosome 8q21.3. OTUD6B belongs to the ovarian tumor (OTU) domain-containing deubiquitinase family, which removes ubiquitin moieties from target proteins to regulate their stability, localization, and signaling activity. OTUD6B plays an important role in cellular protein homeostasis, DNA damage repair, and immune signaling by fine-tuning ubiquitin-mediated pathways. It is expressed in most tissues, with highest levels in brain, liver, and testis, reflecting its broad regulatory functions.

Structurally, OTUD6B contains an N-terminal OTU catalytic domain characterized by a conserved cysteine, histidine, and aspartate catalytic triad responsible for cleaving ubiquitin from substrates. It also includes regions for substrate recognition and potential regulatory phosphorylation sites. OTUD6B belongs to the OTU-type deubiquitinase family, which exhibits linkage-specific cleavage of polyubiquitin chains. Co-localization studies show OTUD6B primarily in the cytoplasm but also associated with the nucleus under stress conditions, suggesting roles in both cytosolic and nuclear signaling pathways.

Functionally, OTUD6B regulates protein degradation and signaling by removing ubiquitin from specific substrates targeted for proteasomal or lysosomal turnover. It modulates the stability of signaling mediators such as AKT, p53, and components of the mTOR pathway. OTUD6B also contributes to endoplasmic reticulum stress responses and DNA damage repair by reversing ubiquitination on repair factors. In immune cells, OTUD6B helps maintain homeostasis by controlling NF-?B activation and cytokine signaling. Known biochemical activities include cleavage of Lys48- and Lys63-linked ubiquitin chains, thereby influencing protein degradation and signaling transduction.

Deficiency or mutation of OTUD6B has been associated with intellectual developmental disorders, craniofacial abnormalities, and growth retardation, collectively known as OTUD6B-related neurodevelopmental syndrome. Dysregulation of OTUD6B expression has also been linked to tumor progression through effects on apoptosis and metabolic control. Pathway associations include ubiquitin-dependent protein catabolism, mTOR signaling, and cellular stress response. During development, OTUD6B supports cell growth and neuronal maturation by regulating key ubiquitinated targets involved in metabolism and transcription.

The OTUD6B antibody from NSJ Bioreagents is an excellent reagent for research on deubiquitination mechanisms, proteostasis, and signal transduction regulation.

UniProt: Q8N6M0