PTCD3 anticorps (AA 66-648)

N° du produit ABIN7876349

Cet anticorps Lapin Polyclonal détecte spécifiquement PTCD3 dans WB, IHC, ELISA et FACS. Il présente une réactivité avec des échantillons de Humain, Souris et Rat.

Aperçu rapide pour PTCD3 anticorps (AA 66-648) (ABIN7876349)

Antigène: PTCD3 (Pentatricopeptide Repeat Domain 3 (PTCD3))

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp PTCD3 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Flow Cytometry (FACS)

Détail du produit anti-PTCD3 anticorps

Épitope: AA 66-648

Fonction: PTCD3 Antibody / Pentatricopeptide repeat domain-containing protein 3

Purification: Immunogen affinity purified

Immunogène: E.coli-derived human PTCD3 recombinant protein (Position: D66-R648) was used as the immunogen for the PTCD3 antibody.

Isotype: IgG

Information d'application

Indications d'application: Optimal dilution of the PTCD3 antibody should be determined by the researcher.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Stock: 4 °C,-20 °C

Stockage commentaire: After reconstitution, the PTCD3 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Détails sur PTCD3

Antigène: PTCD3 (Pentatricopeptide Repeat Domain 3 (PTCD3))

Autre désignation: PTCD3

Sujet: PTCD3 antibody detects Pentatricopeptide repeat domain-containing protein 3, encoded by the PTCD3 gene on chromosome 2p11.2. PTCD3 antibody is used to study this mitochondrial RNA-binding protein required for mitochondrial translation and respiratory function. PTCD3 belongs to the large pentatricopeptide repeat (PPR) protein family, which regulates RNA metabolism in organelles. It is localized to the mitochondrial matrix, where it participates in ribosome assembly and translation initiation for mitochondrial-encoded genes. Expression is ubiquitous but highest in tissues with high metabolic demand such as heart, brain, and muscle.

Structurally, PTCD3 contains multiple PPR motifs that form helix-turn-helix repeats mediating RNA binding. These motifs allow PTCD3 to recognize specific RNA sequences, stabilizing ribosome-mRNA complexes and supporting efficient translation. PTCD3 associates with the small subunit of the mitochondrial ribosome (28S subunit), acting as a structural and functional component of the translation machinery.

Functionally, PTCD3 ensures proper mitochondrial protein synthesis by stabilizing ribosomal complexes and supporting initiation of translation. Knockdown or mutation disrupts translation of mitochondrial mRNAs, leading to impaired oxidative phosphorylation complex assembly and respiratory chain dysfunction. PTCD3 is also thought to regulate specific RNA processing events, although its exact targets remain under investigation. Researchers use PTCD3 antibody to investigate mitochondrial translation, ribosome biology, and energy metabolism.

Clinically, mutations in PTCD3 have been associated with mitochondrial encephalopathy, a disorder characterized by neurological dysfunction, muscle weakness, and lactic acidosis. PTCD3 deficiency results in combined oxidative phosphorylation defects, underscoring its essential role in mitochondrial function. Beyond inherited disorders, PTCD3 expression changes have been reported in cancers, reflecting the importance of mitochondrial activity in tumor metabolism. NSJ Bioreagents offers PTCD3 antibody for studies in mitochondrial biology, disease pathogenesis, and metabolic regulation.

Experimentally, PTCD3 antibody is applied in western blotting to detect the ~79 kDa protein, in immunofluorescence microscopy to confirm mitochondrial localization, and in immunohistochemistry to study tissue-specific expression. Immunoprecipitation with PTCD3 antibody allows isolation of mitochondrial ribosome complexes for functional analysis.

UniProt: Q96EY7