NSMCE4A anticorps (AA 69-380)

N° du produit ABIN7876445

Cet anticorps Lapin Polyclonal détecte spécifiquement NSMCE4A dans WB, ELISA, IF, FACS et ICC. Il présente une réactivité avec des échantillons de Humain, Souris et Rat.

Aperçu rapide pour NSMCE4A anticorps (AA 69-380) (ABIN7876445)

Antigène: NSMCE4A (Non-SMC Element 4 Homolog A (NSMCE4A))

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp NSMCE4A est non-conjugé

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS), Immunocytochemistry (ICC)

Détail du produit anti-NSMCE4A anticorps

Épitope: AA 69-380

Fonction: NSMCE4A Antibody / Non-SMC element 4 homolog A

Purification: Immunogen affinity purified

Immunogène: E.coli-derived human NSMCE4A recombinant protein (Position: D69-Q380) was used as the immunogen for the NSMCE4A antibody.

Isotype: IgG

Information d'application

Indications d'application: Optimal dilution of the NSMCE4A antibody should be determined by the researcher.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Stock: 4 °C,-20 °C

Stockage commentaire: After reconstitution, the NSMCE4A antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Détails sur NSMCE4A

Antigène: NSMCE4A (Non-SMC Element 4 Homolog A (NSMCE4A))

Autre désignation: NSMCE4A

Sujet: NSMCE4A antibody detects Non-structural maintenance of chromosomes element 4A, a core component of the SMC5/6 complex involved in chromosome maintenance, DNA repair, and replication fork stability. Encoded by the NSMCE4A gene on chromosome 10q25.3, this protein forms part of the essential SMC5/6 complex that ensures proper segregation of sister chromatids and preserves genome integrity during cell division and stress. NSMCE4A serves as a bridging subunit, connecting the SMC5 and SMC6 heterodimer to regulatory and enzymatic cofactors that control DNA repair and replication restart.

Structurally, NSMCE4A is a 34 kilodalton nuclear protein that interacts directly with SMC5 and SMC6 through its C-terminal coiled-coil domains, forming a stable hinge component of the complex. Its N-terminal region mediates associations with additional subunits such as NSMCE1 and NSMCE2, which confer ubiquitin ligase activity and DNA damage response signaling capacity. This modular organization enables NSMCE4A to coordinate complex assembly and spatial localization at damaged DNA sites.

The NSMCE4A antibody is widely used in molecular biology, DNA repair, and cell cycle research to study chromosomal maintenance, recombination, and replication dynamics. Western blot analysis identifies a 34 kilodalton band corresponding to NSMCE4A, while immunofluorescence shows punctate nuclear staining that increases following DNA damage. This antibody provides a powerful reagent for investigating the SMC5/6 complex and its role in genome stability.

Functionally, NSMCE4A is required for homologous recombination repair, resolution of DNA replication stress, and suppression of chromosomal rearrangements. Loss of NSMCE4A disrupts complex assembly, leading to DNA double-strand break accumulation and mitotic abnormalities. The SMC5/6 complex, including NSMCE4A, also regulates the maintenance of repetitive DNA sequences and prevents unscheduled recombination at telomeres and ribosomal DNA. Aberrant NSMCE4A expression or mutation has been implicated in genome instability disorders and cancer, where defective repair pathways promote genomic rearrangements. The NSMCE4A antibody enables sensitive detection and quantification of this repair factor in mechanistic studies of DNA integrity, replication stress, and chromosome segregation. NSJ Bioreagents validates this antibody for its applications, ensuring reproducible performance for DNA repair and chromosomal biology applications.

UniProt: Q9NXX6

Pathways: Positive Regulation of Response to DNA Damage Stimulus