STXBP3 anticorps (AA 7-592)

N° du produit ABIN7876509

L’anticorps anti-STXBP3 Polyclonal Lapin est utilisé pour la détection de STXBP3 dans des échantillons de Humain, Souris et Rat. Il a été validé pour WB, ELISA, IHC, IF et FACS.

Aperçu rapide pour STXBP3 anticorps (AA 7-592) (ABIN7876509)

Antigène: STXBP3 (Syntaxin Binding Protein 3 (STXBP3))

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp STXBP3 est non-conjugé

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Détail du produit anti-STXBP3 anticorps

Épitope: AA 7-592

Fonction: STXBP3 Antibody / Syntaxin binding protein 3

Purification: Immunogen affinity purified

Immunogène: E.coli-derived human STXBP3 recombinant protein (Position: E7-E592) was used as the immunogen for the STXBP3 antibody.

Isotype: IgG

Information d'application

Indications d'application: Optimal dilution of the STXBP3 antibody should be determined by the researcher.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Stock: 4 °C,-20 °C

Stockage commentaire: After reconstitution, the STXBP3 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Détails sur STXBP3

Antigène: STXBP3 (Syntaxin Binding Protein 3 (STXBP3))

Autre désignation: STXBP3

Sujet: STXBP3 antibody recognizes Syntaxin binding protein 3, a member of the Sec1/Munc18-like (SM) family of proteins that regulate vesicle fusion and exocytosis. The STXBP3 gene encodes a cytosolic protein also known as Munc18c, which interacts with syntaxins, particularly Syntaxin 4, to mediate docking and fusion of vesicles with the plasma membrane. This interaction is critical for regulated exocytosis in diverse cell types including adipocytes, pancreatic beta cells, neurons, and epithelial cells. By controlling SNARE complex assembly, STXBP3 ensures proper timing of vesicle release during insulin secretion, neurotransmission, and cell-surface trafficking of key receptors and transporters.

STXBP3 plays a central role in glucose homeostasis. In adipose and muscle tissue, Munc18c regulates insulin-stimulated translocation of glucose transporter 4 (GLUT4) to the plasma membrane, an essential step in glucose uptake. Dysregulation or reduced expression of STXBP3 has been associated with insulin resistance and type 2 diabetes. In pancreatic beta cells, STXBP3 interacts with Syntaxin 1A and VAMP2 to influence insulin granule exocytosis. Beyond metabolic regulation, STXBP3 participates in vesicle trafficking in epithelial tissues and contributes to cell polarity maintenance and junctional organization.

The human STXBP3 gene is located on chromosome 1p36.23 and contains multiple alternatively spliced isoforms. Protein structure analysis reveals that STXBP3 comprises three domains that form a closed conformation around its syntaxin partners, preventing premature SNARE complex formation. This inhibitory mechanism ensures vesicles only fuse in response to proper signaling cues. The protein's expression is ubiquitous but enriched in secretory tissues and endocrine glands. STXBP3 has also been implicated in immune responses through regulation of vesicle-mediated cytokine release.

Immunohistochemical staining with STXBP3 antibody demonstrates cytoplasmic localization in skeletal muscle, pancreatic islets, and epithelial cells. It serves as a valuable reagent for studies of insulin signaling, exocytosis, vesicular transport, and metabolic regulation. The STXBP3 antibody from NSJ Bioreagents provides a versatile tool for examining vesicle fusion mechanisms and SNARE complex dynamics across various cellular systems.

UniProt: O00186

Pathways: Hormone Transport