TPBG anticorps (AA 70-420)

N° du produit ABIN7876538

Cet anticorps Lapin Polyclonal détecte spécifiquement TPBG dans WB et ELISA. Il présente une réactivité avec des échantillons de Humain, Souris et Rat.

Aperçu rapide pour TPBG anticorps (AA 70-420) (ABIN7876538)

Antigène: TPBG (Trophoblast Glycoprotein (TPBG))

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp TPBG est non-conjugé

Application: Western Blotting (WB), ELISA

Détail du produit anti-TPBG anticorps

Épitope: AA 70-420

Fonction: TPBG Antibody / Trophoblast glycoprotein

Purification: Immunogen affinity purified

Immunogène: E.coli-derived human TPBG recombinant protein (Position: E70-V420) was used as the immunogen for the TPBG antibody.

Isotype: IgG

Information d'application

Indications d'application: Optimal dilution of the TPBG antibody should be determined by the researcher.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Stock: 4 °C,-20 °C

Stockage commentaire: After reconstitution, the TPBG antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Détails sur TPBG

Antigène: TPBG (Trophoblast Glycoprotein (TPBG))

Autre désignation: TPBG

Sujet: TPBG antibody detects Trophoblast glycoprotein, a highly glycosylated cell surface protein involved in cell adhesion, signal transduction, and immune regulation. Also known as 5T4 oncofetal antigen, TPBG is a type I transmembrane glycoprotein expressed in trophoblasts and upregulated in many carcinomas. The TPBG antibody is used extensively in cancer biology, immunology, and stem cell research to study tumor invasion, metastasis, and cell differentiation mechanisms regulated by this protein.

TPBG is encoded by the TPBG gene located on human chromosome 6q14.1. The mature protein is approximately 420 amino acids long and features an N-terminal signal peptide, several leucine-rich repeats, and an immunoglobulin-like domain in its extracellular region. It is anchored to the plasma membrane through a single transmembrane helix and contains a short cytoplasmic tail. Physiologically, TPBG modulates cell-cell interactions in placental development and embryo implantation, while pathologically, it is re-expressed in many epithelial tumors, where it contributes to epithelial-mesenchymal transition (EMT) and metastatic behavior.

The TPBG antibody is commonly used in immunohistochemistry and flow cytometry to identify tumor cells expressing the 5T4 antigen. Elevated TPBG expression has been reported in lung, ovarian, colorectal, and pancreatic cancers and is associated with poor prognosis and increased invasiveness. The protein influences Wnt/beta-catenin and CXCR4 signaling pathways, promoting migration and stem-like phenotypes. Western blot typically shows a broad band between 72-80 kDa, reflecting heavy glycosylation. Immunofluorescence microscopy reveals localization along the plasma membrane and at cell junctions.

In stem cell research, TPBG expression marks the transition from pluripotency to differentiation, making it a useful biomarker in embryonic stem cell studies. It modulates cytoskeletal dynamics and cell polarity through interactions with intracellular adaptors. Therapeutically, TPBG serves as a target for cancer immunotherapies, including antibody-drug conjugates and vaccines under clinical evaluation. NSJ Bioreagents provides a validated TPBG antibody optimized for western blot, flow cytometry, and immunohistochemistry, enabling reliable detection of this tumor-associated glycoprotein and supporting ongoing efforts in oncology and regenerative medicine research.

UniProt: Q13641