GAD anticorps (AA 72-135)

N° du produit ABIN7876623

L’anticorps anti-GAD Monoclonal Souris est utilisé pour la détection de GAD dans des échantillons de Humain. Il a été validé pour WB, ELISA et IHC (p).

Aperçu rapide pour GAD anticorps (AA 72-135) (ABIN7876623)

Antigène: GAD (GAD1) (Glutamate Decarboxylase 1 (Brain, 67kDa) (GAD1))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp GAD est non-conjugé

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: GAD1-2391

Détail du produit anti-GAD anticorps

Épitope: AA 72-135

Fonction: GAD67 Antibody / GAD1

Purification: Protein G affinity chromatography

Immunogène: A portion of amino acids 72-135 from the human protein were used as the immunogen for this GAD67 antibody.

Isotype: IgG1, kappa

Information d'application

Indications d'application: Optimal dilution of the GAD67 antibody should be determined by the researcher.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store the GAD67 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Détails sur GAD

Antigène: GAD (GAD1) (Glutamate Decarboxylase 1 (Brain, 67kDa) (GAD1))

Autre désignation: GAD67

Sujet: This mAb recognizes a protein of 67 kDa, which is identified as glutamic acid decarboxylase 1 (GDA1). There are two forms of glutamic acid decarboxylases (GADs) that are found in the brain: GAD65 (also known as GAD2) and GAD67 (also known as GAD1). GAD65 and GAD67 are members of the group II decarboxylase family of proteins and are responsible for catalyzing the rate-limiting step in the production of GABA (gamma-aminobutyric acid) from L-glutamic acid. Although both GAD s are found in the brain, GAD65 localizes to synaptic vesicle membranes in nerve terminals, while GAD67 is distributed throughout the cell. GAD67 is responsible for the basal levels of GABA synthesis. In the case of a heightened demand for GABA in neurotransmission, GAD65 will transiently activate to assist in GABA production. The loss of GAD65 is detrimental and can impair GABA neurotransmission, however the loss of GAD67 is lethal.

UniProt: Q99259