NPAT anticorps (AA 87-1191)

N° du produit ABIN7877013

L’anticorps anti-NPAT Polyclonal Lapin est utilisé pour la détection de NPAT dans des échantillons de Humain, Souris et Rat. Il a été validé pour WB, IF, IHC, ELISA, FACS et ICC.

Aperçu rapide pour NPAT anticorps (AA 87-1191) (ABIN7877013)

Antigène: NPAT (Nuclear Protein, Ataxia-Telangiectasia Locus (NPAT))

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp NPAT est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (IHC), ELISA, Flow Cytometry (FACS), Immunocytochemistry (ICC)

Détail du produit anti-NPAT anticorps

Épitope: AA 87-1191

Fonction: NPAT Antibody / Nuclear protein of the ATM locus

Purification: Immunogen affinity purified

Immunogène: E.coli-derived human NPAT recombinant protein (Position: K87-Q1191) was used as the immunogen for the NPAT antibody.

Isotype: IgG

Information d'application

Indications d'application: Optimal dilution of the NPAT antibody should be determined by the researcher.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Stock: 4 °C,-20 °C

Stockage commentaire: After reconstitution, the NPAT antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Détails sur NPAT

Antigène: NPAT (Nuclear Protein, Ataxia-Telangiectasia Locus (NPAT))

Autre désignation: NPAT

Sujet: NPAT antibody detects Nuclear protein, ataxia-telangiectasia locus, encoded by the NPAT gene on chromosome 11q22.3. NPAT antibody is used to study this nuclear regulatory protein, which coordinates cell cycle progression, histone gene transcription, and DNA damage responses. NPAT was identified as a substrate of ATM kinase, linking it to DNA damage signaling and ataxia-telangiectasia biology. It is ubiquitously expressed, with high levels in proliferating cells, particularly in tissues with active cell division such as bone marrow and lymphoid organs.

Structurally, NPAT contains multiple phosphorylation sites targeted by cyclin-dependent kinases (CDKs) and ATM. These modifications regulate its activity during cell cycle transitions and in response to DNA damage. NPAT localizes to Cajal bodies and histone gene clusters, where it acts as a transcriptional co-activator for histone genes. This localization is mediated by its interaction with the transcription factor HiNF-P, forming the HiNF-P/NPAT complex essential for histone gene transcription.

Functionally, NPAT is indispensable for S-phase progression. It activates histone gene transcription at the G1/S transition, ensuring histone supply for DNA replication. NPAT also influences DNA repair by participating in ATM signaling, linking DNA damage sensing to transcriptional regulation. Knockout studies in mice show embryonic lethality, confirming NPAT's essential role in development. Researchers apply NPAT antibody to study its functions in chromatin biology, cell cycle regulation, and DNA repair pathways.

Clinically, NPAT is significant in cancer biology. Overexpression is observed in certain malignancies, where it supports uncontrolled proliferation. Altered NPAT activity may contribute to genomic instability and defective DNA damage responses. Its regulation by ATM connects NPAT to ataxia-telangiectasia, a neurodegenerative and cancer-prone disorder caused by ATM mutations. NPAT is also a potential biomarker of proliferation, with expression correlating to cell cycle activity. NSJ Bioreagents offers NPAT antibody to facilitate studies in DNA replication, chromatin remodeling, and cancer research.

Experimentally, NPAT antibody is used in western blotting to detect the highly phosphorylated 230-260 kDa protein, in immunofluorescence to visualize its localization to histone gene clusters, and in immunohistochemistry to assess proliferation in tissue samples. Immunoprecipitation with NPAT antibody helps characterize its interactions with HiNF-P and ATM kinase. These tools enable detailed exploration of its cell cycle and DNA repair functions.

UniProt: Q14207