NPRL2 anticorps (AA 90-380)

N° du produit ABIN7877107

Cet anticorps anti-NPRL2 Polyclonal Lapin (ABIN7877107) détecte spécifiquement NPRL2 dans WB, ELISA et FACS. L’anticorps est réactif avec des échantillons de Souris et Rat.

Aperçu rapide pour NPRL2 anticorps (AA 90-380) (ABIN7877107)

Antigène: NPRL2 (Nitrogen Permease Regulator-Like 2 (S. Cerevisiae) (NPRL2))

Reactivité: Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp NPRL2 est non-conjugé

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Détail du produit anti-NPRL2 anticorps

Épitope: AA 90-380

Fonction: NPRL2 Antibody / Nitrogen permease regulator-like 2

Purification: Immunogen affinity purified

Immunogène: E.coli-derived human NPRL2 recombinant protein (Position: D90-K380) was used as the immunogen for the NPRL2 antibody.

Isotype: IgG

Information d'application

Indications d'application: Optimal dilution of the NPRL2 antibody should be determined by the researcher.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Stock: 4 °C,-20 °C

Stockage commentaire: After reconstitution, the NPRL2 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Détails sur NPRL2

Antigène: NPRL2 (Nitrogen Permease Regulator-Like 2 (S. Cerevisiae) (NPRL2))

Autre désignation: NPRL2

Sujet: NPRL2 antibody detects Nitrogen permease regulator-like 2, a component of the GATOR1 complex that inhibits mTORC1 signaling in response to amino acid deprivation. NPRL2 functions as a tumor suppressor and metabolic checkpoint regulator, linking nutrient sensing to cell growth and autophagy. The NPRL2 antibody is widely used in metabolism, oncology, and signal transduction research to study mTOR regulation, nutrient signaling, and tumor suppression.

NPRL2 is encoded by the NPRL2 gene located on human chromosome 3p21.3, a region frequently deleted in several cancers. The protein is approximately 380 amino acids long and forms a complex with NPRL3 and DEPDC5 to constitute the GATOR1 inhibitory module. NPRL2 localizes to both cytoplasm and lysosomes, where it acts upstream of Rag GTPases to suppress mTORC1 activity under nutrient-limiting conditions.

The NPRL2 antibody detects a 45 kilodalton band by western blot and reveals punctate cytoplasmic staining consistent with lysosomal localization. NPRL2 is required for proper cellular adaptation to nutrient stress, promoting autophagy and metabolic reprogramming. Loss of NPRL2 results in constitutive mTORC1 activation, uncontrolled cell proliferation, and impaired stress response.

As a tumor suppressor, NPRL2 modulates DNA damage repair and enhances sensitivity to chemotherapeutic agents such as cisplatin. Reduced NPRL2 expression has been documented in lung, colorectal, and breast cancers, correlating with poor prognosis and therapy resistance. Restoring NPRL2 expression reactivates nutrient stress signaling and suppresses tumor growth through mTOR inhibition and apoptosis induction.

Through its dual role in nutrient sensing and cancer suppression, NPRL2 serves as a key regulator of cellular homeostasis. NSJ Bioreagents provides a validated NPRL2 antibody optimized for its applications, supporting research into mTOR signaling, autophagy regulation, and tumor suppression mechanisms.

UniProt: Q8WTW4