PRPF18 anticorps (AA 93-342)

N° du produit ABIN7877181

L’anticorps anti-PRPF18 Polyclonal Lapin est utilisé pour la détection de PRPF18 dans des échantillons de Poisson zèbre (Danio rerio). Il a été validé pour WB.

Aperçu rapide pour PRPF18 anticorps (AA 93-342) (ABIN7877181)

Antigène: PRPF18 (PRP18 Pre-mRNA Processing Factor 18 (PRPF18))

Reactivité: Poisson zèbre (Danio rerio)

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp PRPF18 est non-conjugé

Application: Western Blotting (WB)

Détail du produit anti-PRPF18 anticorps

Épitope: AA 93-342

Fonction: Zebrafish Prpf18 Antibody / Pre-mRNA-splicing factor 18

Purification: Antigen affinity chromatography

Immunogène: E. coli-derived zebrafish Prpf18 recombinant protein (amino acids E93-L342) was used as the immunogen for the Zebrafish Prpf18 antibody.

Isotype: Ig Fraction

Information d'application

Indications d'application: Optimal dilution of the Zebrafish Prpf18 antibody should be determined by the researcher.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Buffer: 0.5 mg/mL if reconstituted with 0.2 mL sterile DI water

Stock: 4 °C,-20 °C

Stockage commentaire: After reconstitution, the Zebrafish Prpf18 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Détails sur PRPF18

Antigène: PRPF18 (PRP18 Pre-mRNA Processing Factor 18 (PRPF18))

Autre désignation: Prpf18

Sujet: Prpf18 (Pre-mRNA processing factor 18) is a key component of the spliceosome, the large ribonucleoprotein complex responsible for the removal of introns from precursor messenger RNA during gene expression. Prpf18 plays an essential role in the second catalytic step of splicing, contributing to the proper assembly and function of the spliceosomal complex and ensuring the accuracy of mRNA processing.

In zebrafish, Prpf18 is an ortholog of the human PRPF18 gene. The zebrafish and human proteins are highly conserved, particularly within domains essential for spliceosomal interactions and catalytic activity. This conservation suggests functional equivalency between species, making zebrafish a relevant model for studying pre-mRNA splicing and its regulation in development and disease.

Zebrafish Prpf18 may have isoforms arising from alternative splicing events, although isoform-specific functions are still under investigation. The presence of multiple isoforms may allow differential regulation of splicing in a tissue-specific or developmental stage-specific manner.

During zebrafish embryogenesis, Prpf18 is broadly expressed, with notable enrichment in rapidly dividing and transcriptionally active tissues such as the neural tube and developing somites. Its expression is crucial for early development, as disruption of splicing machinery can lead to widespread defects in gene regulation, cell cycle control, and differentiation.

Given its central role in RNA splicing, zebrafish Prpf18 is important for studies related to gene expression regulation, developmental biology, and spliceosome-associated disorders. It also serves as a useful model for understanding human genetic diseases caused by splicing defects, including certain neurodegenerative and retinal disorders.

UniProt: Q6GMH0