IRX1 anticorps (AA 95-480)

N° du produit ABIN7877224

Cet anticorps anti-IRX1 Polyclonal Lapin (ABIN7877224) détecte spécifiquement IRX1 dans WB et ELISA. L’anticorps est réactif avec des échantillons de Humain, Souris et Rat.

Aperçu rapide pour IRX1 anticorps (AA 95-480) (ABIN7877224)

Antigène: IRX1 (Iroquois Homeobox Protein 1 (IRX1))

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp IRX1 est non-conjugé

Application: Western Blotting (WB), ELISA

Détail du produit anti-IRX1 anticorps

Épitope: AA 95-480

Fonction: IRX1 Antibody / Iroquois-class homeodomain protein

Purification: Immunogen affinity purified

Immunogène: E.coli-derived human IRX1 recombinant protein (Position: Q95-A480) was used as the immunogen for the IRX1 antibody.

Isotype: IgG

Information d'application

Indications d'application: Optimal dilution of the IRX1 antibody should be determined by the researcher.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Stock: 4 °C,-20 °C

Stockage commentaire: After reconstitution, the IRX1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Détails sur IRX1

Antigène: IRX1 (Iroquois Homeobox Protein 1 (IRX1))

Autre désignation: IRX1

Sujet: IRX1 antibody detects Iroquois-class homeodomain protein IRX1, encoded by the IRX1 gene on chromosome 5p15.33. IRX1 is a transcription factor belonging to the Iroquois homeobox gene family, which regulates embryonic patterning, neuronal differentiation, and organogenesis. IRX1 contains a conserved homeodomain that binds DNA and directs gene expression programs critical for tissue development. In vertebrates, IRX1 contributes to forebrain, heart, limb, and craniofacial patterning. By modulating downstream effectors, it integrates positional cues during embryogenesis, ensuring correct morphogenesis.

IRX1 expression persists in select adult tissues, suggesting roles beyond development, including neuronal plasticity and epithelial regulation. Dysregulation of IRX1 has been linked to oncogenesis. Tumor suppressor roles are observed in gastric cancer, where IRX1 downregulation correlates with poor prognosis, while in other cancers it may function as an oncogene depending on cellular context. Epigenetic silencing of IRX1 through promoter methylation has been reported, highlighting transcriptional repression as a mechanism in cancer progression. IRX1 antibody therefore provides researchers with a means to examine both developmental biology and disease-associated misregulation.

At the molecular level, IRX1 acts as a transcriptional regulator of Wnt, BMP, and Notch signaling pathways, impacting proliferation, differentiation, and apoptosis. In cardiac development, IRX1 contributes to ventricular chamber specification and conduction system maturation. In neural development, it regulates differentiation of specific neuronal subtypes. Animal models with IRX1 knockouts display craniofacial abnormalities, cardiac defects, and disrupted hindbrain segmentation, highlighting its multifaceted role.

Experimentally, IRX1 antibody has applications in immunohistochemistry, immunofluorescence, and western blotting. It is used to track IRX1 expression during embryogenesis, to validate promoter methylation effects in tumors, and to study transcription factor localization in nuclei. NSJ Bioreagents provides IRX1 antibody to facilitate investigations into morphogenetic regulation, cancer epigenetics, and gene regulatory networks.

UniProt: P78414

Pathways: Tube Formation