NUSAP1 anticorps (Middle Region)

N° du produit ABIN7879532

Cet anticorps Lapin Polyclonal détecte spécifiquement NUSAP1 dans WB, IHC, IF, ICC et FACS. Il présente une réactivité avec des échantillons de Humain.

Aperçu rapide pour NUSAP1 anticorps (Middle Region) (ABIN7879532)

Antigène: NUSAP1 (Nucleolar and Spindle Associated Protein 1 (NUSAP1))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp NUSAP1 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Détail du produit anti-NUSAP1 anticorps

Épitope: Middle Region

Fonction: NUSAP1 Antibody / Nucleolar and spindle-associated protein 1

Purification: Immunogen affinity purified

Immunogène: A synthetic peptide corresponding to a sequence in the middle region of human NUSAP1 was used as the immunogen for the NUSAP1 antibody.

Isotype: IgG

Information d'application

Indications d'application: Optimal dilution of the NUSAP1 antibody should be determined by the researcher.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Stock: 4 °C,-20 °C

Stockage commentaire: After reconstitution, the NUSAP1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Détails sur NUSAP1

Antigène: NUSAP1 (Nucleolar and Spindle Associated Protein 1 (NUSAP1))

Autre désignation: NUSAP1

Sujet: NUSAP1 antibody detects Nucleolar and spindle-associated protein 1, a microtubule-binding protein essential for spindle formation and chromosome segregation during mitosis. The UniProt recommended name is Nucleolar and spindle-associated protein 1 (NUSAP1). This mitotic regulator links chromatin to microtubules, ensuring proper spindle assembly and stabilization required for accurate chromosome alignment and segregation.

Functionally, NUSAP1 antibody identifies a 495-amino-acid nuclear protein that localizes to the mitotic spindle and spindle poles. NUSAP1 binds microtubules through its C-terminal domain and bundles them into stable arrays, promoting bipolar spindle formation. It also interacts with chromatin components, including DNA and nucleolar proteins, to coordinate chromosome condensation and segregation during metaphase and anaphase.

The NUSAP1 gene, located on chromosome 15q15.1, encodes a cell cycle-regulated protein that peaks in expression during G2/M phase and is degraded after mitosis. NUSAP1 is phosphorylated by CDK1, controlling its localization and activity. It functions in concert with kinesin and dynein motors, contributing to spindle microtubule dynamics and mitotic checkpoint signaling.

Pathologically, elevated NUSAP1 expression is a hallmark of proliferating cells and various cancers, including breast, prostate, and lung carcinoma. Its overexpression correlates with increased tumor aggressiveness and poor prognosis, reflecting enhanced proliferative capacity. Conversely, NUSAP1 depletion leads to spindle defects, mitotic arrest, and genomic instability. Research with NUSAP1 antibody provides insight into cell division mechanisms and cancer cell cycle regulation.

NUSAP1 antibody is suitable for use in western blotting, immunofluorescence, and immunohistochemistry to detect mitotic spindles and cell cycle progression. NSJ Bioreagents supplies validated NUSAP1 antibody reagents ideal for studies of mitosis, chromosomal segregation, and cancer cell biology.

Structurally, NUSAP1 contains a coiled-coil domain, a microtubule-binding motif, and a DNA-binding region, which together mediate its dual role in chromatin attachment and microtubule bundling. This antibody supports exploration of NUSAP1i 1/2s role as a spindle scaffold that integrates chromosomal and cytoskeletal control during mitosis.

UniProt: Q9BXS6