PABPN1 anticorps (Middle Region)

N° du produit ABIN7879541

Cet anticorps Lapin Polyclonal détecte spécifiquement PABPN1 dans WB, IF, IHC, IP, ICC et FACS. Il présente une réactivité avec des échantillons de Humain, Souris et Rat.

Aperçu rapide pour PABPN1 anticorps (Middle Region) (ABIN7879541)

Antigène: PABPN1 (Poly A Binding Protein Nuclear 1 (PABPN1))

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp PABPN1 est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Détail du produit anti-PABPN1 anticorps

Épitope: Middle Region

Fonction: PABPN1 Antibody / Polyadenylate-binding protein 2

Purification: Immunogen affinity purified

Immunogène: A synthetic peptide corresponding to a sequence in the middle region of human PABPN1 was used as the immunogen for the PABPN1 antibody.

Isotype: IgG

Information d'application

Indications d'application: Optimal dilution of the PABPN1 antibody should be determined by the researcher.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Stock: 4 °C,-20 °C

Stockage commentaire: After reconstitution, the PABPN1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Détails sur PABPN1

Antigène: PABPN1 (Poly A Binding Protein Nuclear 1 (PABPN1))

Autre désignation: PABPN1

Sujet: PABPN1 antibody detects Polyadenylate-binding protein 2, encoded by the PABPN1 gene. Polyadenylate-binding protein 2 is a nuclear protein that binds to the poly(A) tail of RNA and regulates mRNA processing, stability, and translation. PABPN1 antibody provides researchers with a critical reagent for studying RNA metabolism, gene expression, and human disease associated with RNA dysregulation.

Polyadenylate-binding protein 2 is essential for the regulation of poly(A) tail length and stability of nuclear transcripts. Research using PABPN1 antibody has shown that it stimulates poly(A) polymerase activity and ensures that poly(A) tails reach optimal length for nuclear export and translation. By binding RNA, PABPN1 prevents premature deadenylation and degradation, thereby safeguarding mRNA function.

Studies with PABPN1 antibody have revealed that mutations in PABPN1 cause oculopharyngeal muscular dystrophy, a late-onset muscle disorder characterized by progressive weakness of eyelid, pharyngeal, and proximal limb muscles. The disease results from short expansions of a GCN trinucleotide repeat in the gene, which cause protein aggregation and dysfunction. This clinical connection underscores the importance of PABPN1 in human health.

Beyond muscular dystrophy, research using PABPN1 antibody has shown that the protein regulates alternative polyadenylation, influencing transcript diversity and gene expression patterns. Dysregulation of polyadenylation contributes to cancer, neurodegeneration, and developmental disorders, making PABPN1 a key factor in RNA biology.

PABPN1 antibody is widely applied in western blotting, immunohistochemistry, and RNA immunoprecipitation. Western blotting quantifies protein levels in muscle and brain tissue, immunohistochemistry demonstrates nuclear localization, and RNA immunoprecipitation identifies target transcripts. These applications make PABPN1 antibody indispensable for RNA processing research.

By providing validated PABPN1 antibody reagents, NSJ Bioreagents supports studies into polyadenylation, muscular dystrophy, and RNA regulation. Detection of Polyadenylate-binding protein 2 provides researchers with insight into how nuclear RNA-binding proteins regulate transcript stability and disease.

UniProt: Q86U42