GK2 anticorps (N-Term)

N° du produit ABIN7879604

Cet anticorps Lapin Polyclonal détecte spécifiquement GK2 dans WB, IF, ICC et FACS. Il présente une réactivité avec des échantillons de Humain, Rat et Souris.

Aperçu rapide pour GK2 anticorps (N-Term) (ABIN7879604)

Antigène: GK2 (Glycerol Kinase 2 (GK2))

Reactivité: Humain, Rat, Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp GK2 est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Détail du produit anti-GK2 anticorps

Épitope: N-Term

Fonction: GK2 Antibody / Glycerol kinase 2

Purification: Immunogen affinity purified

Immunogène: A synthetic peptide corresponding to a sequence at the N-terminus of human GK2 was used as the immunogen for the GK2 antibody.

Isotype: IgG

Information d'application

Indications d'application: Optimal dilution of the GK2 antibody should be determined by the researcher.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Stock: 4 °C,-20 °C

Stockage commentaire: After reconstitution, the GK2 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Détails sur GK2

Antigène: GK2 (Glycerol Kinase 2 (GK2))

Autre désignation: GK2

Sujet: GK2 antibody detects Glycerol kinase 2, encoded by the GK2 gene on chromosome 4q13.3. GK2 antibody is commonly applied in studies of glycerol metabolism, mitochondrial energy regulation, and sperm function. GK2 belongs to the glycerol kinase family, enzymes that phosphorylate glycerol to glycerol-3-phosphate, a critical intermediate in lipid metabolism and glycolysis. Unlike the ubiquitously expressed GK1, GK2 is primarily expressed in testis and sperm, where it plays a role in fertility and energy production. It localizes to mitochondria, highlighting its role in coupling glycerol metabolism with energy pathways.

Structurally, GK2 is a ~61 kDa enzyme composed of conserved ATP-binding and substrate-binding domains typical of kinases in this family. It lacks some regulatory motifs present in GK1, leading to distinct enzymatic properties and tissue specificity. The mitochondrial localization sequence targets GK2 to the inner membrane, enabling direct integration into oxidative phosphorylation networks.

Functionally, GK2 catalyzes the phosphorylation of glycerol to glycerol-3-phosphate, fueling glycolysis and triglyceride synthesis. In sperm, this activity supports energy metabolism necessary for motility and fertilization. GK2 also contributes to mitochondrial glycerol-3-phosphate shuttle activity, linking cytosolic NADH reoxidation with the respiratory chain. Knockdown or disruption of GK2 leads to impaired sperm motility and male infertility, emphasizing its critical reproductive role. Researchers use GK2 antibody to study sperm metabolism, mitochondrial function, and lipid biochemistry.

Clinically, GK2 mutations or dysfunction have been associated with infertility and metabolic syndromes involving lipid dysregulation. Because GK2 expression is highly specific to testis, it has been investigated as a biomarker of male fertility potential. GK2 is also implicated in mitochondrial biology disorders, where energy coupling is impaired. Its restricted expression compared to GK1 makes it an attractive target for reproductive health research. NSJ Bioreagents provides GK2 antibody as a validated tool for mitochondrial and fertility research.

Experimentally, GK2 antibody is applied in western blotting to detect the ~61 kDa protein, in immunofluorescence microscopy to localize it in mitochondria, and in immunohistochemistry to examine expression in testis tissue. Enzyme assays combined with GK2 antibody detection enable correlation of enzymatic activity with localization and expression.

UniProt: Q14410