ODF1 anticorps (N-Term)

N° du produit ABIN7879639

Cet anticorps anti-ODF1 Polyclonal Lapin (ABIN7879639) détecte spécifiquement ODF1 dans WB et FACS. L’anticorps est réactif avec des échantillons de Humain, Souris et Rat.

Aperçu rapide pour ODF1 anticorps (N-Term) (ABIN7879639)

Antigène: ODF1 (Outer Dense Fiber of Sperm Tails 1 (ODF1))

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp ODF1 est non-conjugé

Application: Western Blotting (WB), Flow Cytometry (FACS)

Détail du produit anti-ODF1 anticorps

Épitope: N-Term

Fonction: ODF1 Antibody / Outer dense fiber protein 1

Purification: Immunogen affinity purified

Immunogène: A synthetic peptide corresponding to a sequence at the N-terminus of human ODF1 was used as the immunogen for the ODF1 antibody.

Isotype: IgG

Information d'application

Indications d'application: Optimal dilution of the ODF1 antibody should be determined by the researcher.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Stock: 4 °C,-20 °C

Stockage commentaire: After reconstitution, the ODF1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Détails sur ODF1

Antigène: ODF1 (Outer Dense Fiber of Sperm Tails 1 (ODF1))

Autre désignation: ODF1

Sujet: ODF1 antibody detects Outer dense fiber protein 1, encoded by the ODF1 gene on chromosome 8q22.2. ODF1 antibody is widely applied in reproductive biology, spermatogenesis, and fertility research. ODF1 is a structural protein of the sperm tail, specifically localized to the outer dense fibers that run alongside the axoneme in the principal piece of the flagellum. These fibers provide mechanical strength, protect the axoneme from shear stress, and contribute to the elastic recoil properties of sperm movement.

Structurally, ODF1 is a ~27 kDa coiled-coil protein with leucine zipper motifs that promote oligomerization. It is one of the earliest proteins incorporated into outer dense fibers during spermatid differentiation. ODF1 interacts with other outer dense fiber proteins such as ODF2 and with axonemal microtubules, forming the cytoskeletal backbone of the sperm tail.

Functionally, ODF1 contributes to sperm motility by reinforcing the axoneme and supporting energy transmission along the flagellum. Loss of ODF1 leads to structural abnormalities in the sperm tail and impaired motility, resulting in infertility. Researchers use ODF1 antibody to study spermatogenesis, sperm structure, and male fertility.

Clinically, ODF1 mutations or dysregulation have been associated with asthenozoospermia and male infertility. Studies in mice show that ODF1 knockout results in male sterility due to defective sperm tails. Because sperm tail defects are a common cause of infertility, ODF1 is a biomarker of reproductive health. NSJ Bioreagents provides ODF1 antibody for fertility and reproductive biology research.

Experimentally, ODF1 antibody is used in western blotting to detect the ~27 kDa protein, in immunofluorescence to visualize sperm tail localization, and in immunohistochemistry of testicular sections. Co-immunoprecipitation with ODF1 antibody reveals interactions with cytoskeletal partners critical for flagellar integrity.

UniProt: Q14990