Pcp2 anticorps (N-Term)

N° du produit ABIN7879647

L’anticorps anti-Pcp2 Polyclonal Lapin est utilisé pour la détection de Pcp2 dans des échantillons de Souris, Humain et Rat. Il a été validé pour WB.

Aperçu rapide pour Pcp2 anticorps (N-Term) (ABIN7879647)

Antigène: Pcp2 (PCP2) (Purkinje Cell Protein 2 (PCP2))

Reactivité: Souris, Humain, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Pcp2 est non-conjugé

Application: Western Blotting (WB)

Détail du produit anti-Pcp2 anticorps

Épitope: N-Term

Fonction: PCP2 Antibody / Purkinje cell protein 2

Purification: Immunogen affinity purified

Immunogène: A synthetic peptide corresponding to a sequence at the N-terminus of human PCP2 was used as the immunogen for the PCP2 antibody.

Isotype: IgG

Information d'application

Indications d'application: Optimal dilution of the PCP2 antibody should be determined by the researcher.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Stock: 4 °C,-20 °C

Stockage commentaire: After reconstitution, the PCP2 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Détails sur Pcp2

Antigène: Pcp2 (PCP2) (Purkinje Cell Protein 2 (PCP2))

Autre désignation: PCP2

Sujet: PCP2 antibody detects Purkinje cell protein 2, a brain-specific regulator of G protein signaling and neuronal function. Encoded by the PCP2 gene on chromosome 19p13.2, this protein is selectively expressed in cerebellar Purkinje cells and retinal bipolar neurons. PCP2, also known as L7, modulates intracellular signaling by binding to Galpha subunits of heterotrimeric G proteins, influencing synaptic transmission and motor coordination.

PCP2 contains a GoLoco motif that enables high-affinity binding to inactive GDP-bound Galpha subunits, acting as a guanine nucleotide dissociation inhibitor (GDI). Through this interaction, it regulates G protein-coupled receptor (GPCR) signaling pathways that affect neuronal excitability and neurotransmitter release. PCP2 expression increases during cerebellar development, coinciding with synapse formation and maturation of Purkinje cells.

The PCP2 antibody is widely used in neurobiology, developmental, and signaling research to investigate cerebellar function, G protein regulation, and neuronal connectivity. Western blot analysis identifies a 17 kilodalton band corresponding to PCP2, while immunohistochemistry shows intense staining in Purkinje cell somata and dendrites, as well as retinal bipolar neurons. This antibody enables precise visualization of neuronal subtypes and signaling dynamics in the cerebellum and retina.

Altered expression of PCP2 has been associated with ataxia, autism spectrum disorders, and visual processing abnormalities, reflecting its role in synaptic signal modulation. The PCP2 antibody provides a sensitive tool for exploring cerebellar physiology and G protein regulation. NSJ Bioreagents validates this antibody for its applications, ensuring robust detection for neuroscience applications.

UniProt: Q8IVA1