Recombinant MPRIP anticorps (pSer232)

N° du produit ABIN7879721

Cet anticorps Lapin Monoclonal détecte spécifiquement MPRIP dans WB. Il présente une réactivité avec des échantillons de Souris et Rat.

Aperçu rapide pour Recombinant MPRIP anticorps (pSer232) (ABIN7879721)

Antigène: MPRIP (Myosin Phosphatase rho Interacting Protein (MPRIP))

Type d'anticorp: Recombinant Antibody

Reactivité: Souris, Rat

Hôte: Lapin

Clonalité: Monoclonal

Conjugué: Cet anticorp MPRIP est non-conjugé

Application: Western Blotting (WB)

Clone: 31R36

Détail du produit anti-MPRIP anticorps

Épitope: pSer232

Fonction: Phospho-RIP3 (Ser232) Antibody / RIPK3

Purification: Affinity chromatography

Immunogène: A synthesized peptide derived from human Phospho-RIP3 (S232) was used as the immunogen for the Phospho-RIP3 (Ser232) antibody.

Isotype: IgG

Information d'application

Indications d'application: Optimal dilution of the Phospho-RIP3 (Ser232) antibody should be determined by the researcher.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: Rabbit IgG in phosphate buffered saline, pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol, 0.4-0.5 mg/mL BSA

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store the Phospho-RIP3 (Ser232) antibody at -20oC.

Détails sur MPRIP

Antigène: MPRIP (Myosin Phosphatase rho Interacting Protein (MPRIP))

Autre désignation: RIP3

Sujet: Phospho-RIP3 (Ser232) antibody detects RIP3 phosphorylated at serine 232, encoded by the RIPK3 gene. RIP3, also called Receptor interacting serine threonine kinase 3, is a critical mediator of necroptosis, a regulated form of necrotic cell death. Phospho-RIP3 (Ser232) antibody provides researchers with a specific reagent to study necroptotic signaling, inflammation, and disease mechanisms involving programmed necrosis.

RIP3 functions as a core signaling kinase within the necrosome complex. Research using Phospho-RIP3 (Ser232) antibody has shown that phosphorylation at Ser232 is essential for RIP3 activation and subsequent recruitment of MLKL, the executioner protein of necroptosis. This phosphorylation serves as a molecular switch controlling progression of the necroptotic pathway.

Studies with Phospho-RIP3 (Ser232) antibody have revealed that necroptosis is triggered by tumor necrosis factor signaling, viral infection, and cellular stress conditions. RIP3 phosphorylation at Ser232 enables necrosome assembly, MLKL oligomerization, and disruption of plasma membrane integrity. This process releases intracellular contents that amplify inflammation and immune responses.

In disease contexts, dysregulated necroptosis contributes to pathology. Research using Phospho-RIP3 (Ser232) antibody has shown that excessive activation promotes tissue damage in conditions such as ischemia reperfusion injury, inflammatory bowel disease, and neurodegeneration. Conversely, insufficient necroptosis facilitates viral persistence and impaired immune clearance. These findings highlight RIP3 phosphorylation as a therapeutic target for modulating necroptotic signaling.

Phospho-RIP3 (Ser232) antibody is widely applied in western blotting, immunohistochemistry, and immunofluorescence. Western blotting distinguishes phosphorylated RIP3 from total levels, immunohistochemistry localizes activated necroptotic signaling in tissue, and immunofluorescence reveals subcellular distribution during necrosome formation. These applications make the antibody valuable in cell death research.

By providing validated Phospho-RIP3 (Ser232) antibody reagents, NSJ Bioreagents supports studies into programmed necrosis, inflammation, and therapeutic modulation. Detection of RIP3 phosphorylated at Ser232 provides insight into the molecular regulation of necroptosis and its contribution to disease.

UniProt: Q9Y572