Recombinant TBC1D4 anticorps (pThr642)

N° du produit ABIN7879804

Cet anticorps anti-TBC1D4 Monoclonal Lapin (Clone 31T47) (ABIN7879804) détecte spécifiquement TBC1D4 dans WB. L’anticorps est réactif avec des échantillons de Humain.

Aperçu rapide pour Recombinant TBC1D4 anticorps (pThr642) (ABIN7879804)

Antigène: TBC1D4 (TBC1 Domain Family, Member 4 (TBC1D4))

Type d'anticorp: Recombinant Antibody

Reactivité: Humain

Hôte: Lapin

Clonalité: Monoclonal

Conjugué: Cet anticorp TBC1D4 est non-conjugé

Application: Western Blotting (WB)

Clone: 31T47

Détail du produit anti-TBC1D4 anticorps

Épitope: pThr642

Fonction: Phospho-AS160 (Thr642) Antibody / TBC1D4

Purification: Affinity-chromatography

Immunogène: A synthesized peptide derived from human Phospho-AS160 (T642) was used as the immunogen for the Phospho-AS160 (Thr642) antibody.

Isotype: IgG

Information d'application

Indications d'application: Optimal dilution of the Phospho-AS160 (Thr642) antibody should be determined by the researcher.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: Rabbit IgG in phosphate buffered saline, pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol, 0.4-0.5 mg/mL BSA

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store the Phospho-AS160 (Thr642) antibody at -20oC.

Détails sur TBC1D4

Antigène: TBC1D4 (TBC1 Domain Family, Member 4 (TBC1D4))

Autre désignation: AS160

Sujet: Phospho-AS160 (Thr642) antibody detects TBC1 domain family member 4 when phosphorylated at threonine 642. This protein is encoded by the TBC1D4 gene and is commonly referred to as AS160, Akt substrate of 160 kDa, and insulin-regulated Rab GTPase-activating protein. TBC1D4 functions as a Rab GAP that regulates trafficking of GLUT4 vesicles in response to insulin signaling. Phosphorylation at Thr642 by Akt relieves its inhibitory GAP activity, allowing Rab GTPases to promote translocation of GLUT4 to the plasma membrane, a critical event in glucose uptake by adipocytes and muscle cells.

Phospho-AS160 (Thr642) antibody is widely applied in metabolism, endocrinology, and diabetes research. Detecting phosphorylation at Thr642 provides a direct readout of insulin-stimulated Akt signaling and GLUT4 trafficking. This makes the antibody valuable for studies of insulin resistance, obesity, and type 2 diabetes. In addition, TBC1D4 is expressed in other tissues, linking it to energy homeostasis beyond classical insulin-responsive cells.

Applications for Phospho-AS160 (Thr642) antibody include western blotting, immunohistochemistry, immunofluorescence, and ELISA. Western blot assays detect phosphorylation changes in response to insulin or exercise, immunohistochemistry maps expression in metabolic tissues, and immunofluorescence highlights vesicle trafficking events. These methods allow researchers to connect molecular signaling to physiological glucose uptake.

Dysregulation of TBC1D4 phosphorylation is linked to insulin resistance and diabetes. Reduced phosphorylation impairs GLUT4 trafficking, while mutations in TBC1D4 cause severe postprandial hyperinsulinemia and muscle glycogen storage abnormalities. By applying Phospho-AS160 (Thr642) antibody, scientists can study both common and rare metabolic disorders.

Beyond metabolism, TBC1D4 regulates vesicle trafficking in immune cells, neurons, and other systems. Phosphorylation-dependent regulation highlights its integration with Akt signaling in multiple contexts. NSJ Bioreagents provides Phospho-AS160 (Thr642) antibody with validated specificity, ensuring accurate detection of this phosphorylation event in insulin and trafficking research.

UniProt: O60343