Recombinant SYK anticorps (pTyr323)

N° du produit ABIN7879832

Cet anticorps Lapin Monoclonal détecte spécifiquement SYK dans WB, IHC, IF et ICC. Il présente une réactivité avec des échantillons de Humain.

Aperçu rapide pour Recombinant SYK anticorps (pTyr323) (ABIN7879832)

Antigène: SYK (Spleen tyrosine Kinase (SYK))

Type d'anticorp: Recombinant Antibody

Reactivité: Humain

Hôte: Lapin

Clonalité: Monoclonal

Conjugué: Cet anticorp SYK est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

Clone: 32S11

Détail du produit anti-SYK anticorps

Épitope: pTyr323

Fonction: Phospho-Syk (Tyr323) Antibody / SYK

Purification: Affinity-chromatography

Immunogène: A synthesized peptide derived from human Phospho-Syk (Y323) was used as the immunogen for the Phospho-Syk (Tyr323) antibody.

Isotype: IgG

Information d'application

Indications d'application: Optimal dilution of the Phospho-Syk (Tyr323) antibody should be determined by the researcher.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: Rabbit IgG in phosphate buffered saline, pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol, 0.4-0.5 mg/mL BSA

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store the Phospho-Syk (Tyr323) antibody at -20oC.

Détails sur SYK

Antigène: SYK (Spleen tyrosine Kinase (SYK))

Autre désignation: Syk

Sujet: Phospho-Syk (Tyr323) antibody detects Spleen tyrosine kinase phosphorylated at tyrosine 323, encoded by the SYK gene. Spleen tyrosine kinase is a cytoplasmic non receptor tyrosine kinase that mediates signaling downstream of immunoreceptors such as the B cell receptor, Fc receptors, and integrins. Phosphorylation at tyrosine 323 is a critical modification that regulates kinase activity and adaptor interactions. Phospho-Syk (Tyr323) antibody provides a selective tool for studying immune signaling, B cell activation, and inflammatory responses.

Spleen tyrosine kinase is activated when immunoreceptors are crosslinked, leading to phosphorylation of ITAM motifs and recruitment of SYK through its tandem SH2 domains. Once engaged, SYK becomes phosphorylated at multiple sites, including tyrosine 323, which enhances its catalytic activity and binding to signaling partners. Research with Phospho-Syk (Tyr323) antibody has demonstrated that this phosphorylation event promotes propagation of signaling cascades leading to calcium flux, MAP kinase activation, and transcriptional responses. These events are fundamental for lymphocyte activation and immune defense.

Beyond lymphocytes, SYK plays roles in innate immune cells, including macrophages, mast cells, and neutrophils. Studies with Phospho-Syk (Tyr323) antibody have shown that phosphorylation at this site regulates Fc receptor signaling, phagocytosis, and cytokine release. Dysregulation of SYK phosphorylation has been linked to autoimmune disease, allergy, and chronic inflammation, making it a therapeutic target. Inhibitors of SYK are in clinical development for treatment of rheumatoid arthritis, lupus, and hematologic malignancies.

Phosphorylation at tyrosine 323 also contributes to oncogenic signaling. Research with Phospho-Syk (Tyr323) antibody has revealed aberrant activation in leukemias and lymphomas, where it drives proliferation and survival. Monitoring phosphorylation state is therefore critical for understanding pathogenesis and evaluating targeted therapies. Because SYK functions at the intersection of immunity and cancer, its phosphorylation events remain of high biomedical importance.

Phospho-Syk (Tyr323) antibody is used in western blotting, immunohistochemistry, and flow cytometry. Western blotting distinguishes phosphorylated and unphosphorylated forms, immunohistochemistry localizes activated kinase in tissue samples, and flow cytometry quantifies phosphorylation dynamics in immune cells. These approaches make Phospho-Syk (Tyr323) antibody essential for dissecting immune receptor signaling.

By supplying validated Phospho-Syk (Tyr323) antibody reagents, NSJ Bioreagents supports research into immune signaling, autoimmunity, and cancer. Detection of Spleen tyrosine kinase phosphorylation at tyrosine 323 provides a precise measure of immune activation.

UniProt: P43405

Pathways: Fc-epsilon Receptor Signaling Pathway, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Thromboxane A2 Receptor Signaling, BCR Signaling