ANO1 anticorps

N° du produit ABIN7879983

L’anticorps anti-ANO1 Monoclonal Souris est utilisé pour la détection de ANO1 dans des échantillons de Humain. Il a été validé pour IHC (p).

Aperçu rapide pour ANO1 anticorps (ABIN7879983)

Antigène: ANO1 (Anoctamin 1, Calcium Activated Chloride Channel (ANO1))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp ANO1 est non-conjugé

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: SPM580

Détail du produit anti-ANO1 anticorps

Fonction: ANO1 Antibody / DOG1 / TMEM16A

Purification: Protein G affinity chromatography

Immunogène: Recombinant human DOG1 protein was used as the immunogen for this ANO1 antibody.

Isotype: IgG1, kappa

Information d'application

Indications d'application: The optimal dilution of the ANO1 antibody for each application should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10 mM Tris with 1 mM EDTA for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: Prediluted in 1X PBS, 0.1 mg/mL BSA (US sourced), 0.05 % sodium azide, *For IHC use only*

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store the ANO1 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Détails sur ANO1

Antigène: ANO1 (Anoctamin 1, Calcium Activated Chloride Channel (ANO1))

Autre désignation: ANO1

Sujet: Expression of DOG-1/ANO1/TMEM16A protein is elevated in the gastrointestinal stromal tumors (GISTs), c-kit signaling-driven mesenchymal tumors of the GI tract. ANO1 is rarely expressed in other soft tissue tumors, which, due to appearance, may be difficult to diagnose. Immunoreactivity has been reported in 97.8 percent of scorable GISTs, including all c-kit negative GISTs. Overexpression has been suggested to aid in the identification of GISTs, including Platelet-Derived Growth Factor Receptor Alpha mutants that fail to express c-kit antigen. The overall sensitivity of DOG-1/ANO1 and c-kit in GISTs is nearly identical: 94.4 % vs. 94.7 %.

UniProt: Q5XXA6