Recombinant EIF1AX anticorps

N° du produit ABIN7880747

Cet anticorps Lapin Monoclonal détecte spécifiquement EIF1AX dans IHC, WB, IF, ICC, FACS et IP. Il présente une réactivité avec des échantillons de Humain, Souris et Rat.

Aperçu rapide pour Recombinant EIF1AX anticorps (ABIN7880747)

Antigène: EIF1AX (Eukaryotic Translation Initiation Factor 1A, X-Linked (EIF1AX))

Type d'anticorp: Recombinant Antibody

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Monoclonal

Conjugué: Cet anticorp EIF1AX est non-conjugé

Application: Immunohistochemistry (IHC), Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunoprecipitation (IP)

Clone: 29E78

Détail du produit anti-EIF1AX anticorps

Fonction: EIF1AX Antibody / eIF1A X isoform

Purification: Affinity-chromatography

Immunogène: A synthesized peptide derived from human eIF1A was used as the immunogen for the EIF1AX antibody.

Isotype: IgG

Information d'application

Indications d'application: Optimal dilution of the EIF1AX antibody should be determined by the researcher.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: Rabbit IgG in phosphate buffered saline, pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol, 0.4-0.5 mg/mL BSA

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store the EIF1AX antibody at -20oC.

Détails sur EIF1AX

Antigène: EIF1AX (Eukaryotic Translation Initiation Factor 1A, X-Linked (EIF1AX))

Autre désignation: EIF1AX

Sujet: EIF1AX antibody detects eukaryotic translation initiation factor 1A X linked isoform, encoded by the EIF1AX gene. Common names include eIF1A X isoform, translation initiation factor SUI1 homolog, and X linked eukaryotic translation initiation factor 1A. EIF1AX is a small but critical initiation factor that promotes scanning of mRNA and start codon recognition during translation initiation. It interacts with the 40S ribosomal subunit and other initiation factors to assemble the translation preinitiation complex, ensuring accurate start site selection and efficient protein synthesis.

EIF1AX antibody is widely applied in molecular biology, cancer research, and developmental studies. Translation initiation is a highly regulated step in gene expression, and EIF1AX ensures fidelity in AUG codon selection. Mutations in EIF1AX have been identified in uveal melanoma, thyroid cancer, and endometrial carcinoma, where they cooperate with other oncogenic events. By detecting EIF1AX, researchers can study how altered initiation contributes to tumor biology and translational control.

Applications for EIF1AX antibody include western blotting, immunohistochemistry, and immunofluorescence. Western blotting detects EIF1AX expression in cell lysates, immunohistochemistry maps its expression in tumors and tissues, and immunofluorescence highlights nuclear and cytoplasmic localization. These assays provide strong tools to evaluate translation initiation factor biology in both normal and disease contexts.

EIF1AX mutations often cluster at the N terminus, affecting interactions with other initiation factors and altering start codon selection. Dysregulation of initiation leads to abnormal gene expression patterns, contributing to cell proliferation and tumorigenesis. By applying EIF1AX antibody, scientists can study these processes in detail and evaluate EIF1AX as a potential biomarker or therapeutic target.

EIF1AX also functions in normal development, where accurate translation initiation is critical for embryogenesis and tissue growth. Its conservation across species highlights its fundamental role in biology. The antibody therefore supports research from basic ribosome biology to applied cancer translational studies.

UniProt: P47813