ABO anticorps

N° du produit ABIN7881056

Cet anticorps Souris Monoclonal détecte spécifiquement ABO dans IF. Il présente une réactivité avec des échantillons de Humain.

Aperçu rapide pour ABO anticorps (ABIN7881056)

Antigène: ABO (Histo-blood group A transferase (ABO))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp ABO est non-conjugé

Application: Immunofluorescence (IF)

Clone: A46-B-B10

Détail du produit anti-ABO anticorps

Fonction: ABO Antibody / Blood Group Antigen H Type 2

Purification: Protein A/G affinity

Immunogène: Human breast cancer MCF-7 cells were used as the immunogen for the ABO antibody.

Isotype: IgM, kappa

Information d'application

Indications d'application: Optimal dilution of the ABO antibody should be determined by the researcher.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Aliquot the ABO antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Détails sur ABO

Antigène: ABO (Histo-blood group A transferase (ABO))

Autre désignation: ABO

Sujet: Recognizes the blood group H type 2 antigens, trisaccharide Fuc1 of human origin. The specificity of this MAb has been identified by haemagglutination tests, immunohistochemistry, binding inhibition studies, and absorption experiments performed with synthetic oligosaccharides. The antibody is virtually nonreactive with H type 1 antigen or with closely related type 2 structures (e.g., Y antigen). A46-B/B10 strongly agglutinates human erythrocytes according to the amount of H substance expressed and can, therefore, easily discriminate between blood groups A1 and A2 as well as A1B and A2B (A1 and A1B are not or only weakly agglutinated). In immunohistochemistry, this antibody seems to provide a highly specific reagent for a restricted number of carcinomas and epithelial lineages in tissue sections and in vitro. This was tested on 753 blood samples. It strongly agglutinates human erythrocytes of blood groups 0, A2 and A2B, but not or only very weakly A1 and A1B. This MAb promises to be useful in the determination of A and AB subgroups in blood group serology. This MAb might also be able to define B subgroups. Among 263 B blood samples, 45 (17.1 % ) were negative or only weakly positive with A46-B/B10.

UniProt: P16442