Recombinant Histone H1 anticorps

N° du produit ABIN7881061

Cet anticorps anti-Histone H1 Monoclonal Lapin (Clone AE-4) (ABIN7881061) détecte spécifiquement Histone H1 dans WB, IF, FACS et IHC (p). L’anticorps est réactif avec des échantillons de Humain, Souris et Rat.

Aperçu rapide pour Recombinant Histone H1 anticorps (ABIN7881061)

Antigène: Histone H1

Type d'anticorp: Recombinant Antibody

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Monoclonal

Conjugué: Cet anticorp Histone H1 est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Classe de qualité: Carrier-free

Clone: AE-4

Détail du produit anti-Histone H1 anticorps

Fonction: Recombinant Histone H1 Antibody (azide and preservative free)

Purification: Protein A affinity chromatography

Immunogène: Nuclei of human leukemia biopsy cells were used as the immunogen for this recombinant Histone H1 antibody.

Isotype: IgG

Information d'application

Indications d'application: Optimal dilution of the recombinant Histone H1 antibody should be determined by the researcher.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Agent conservateur: Azide free

Stock: 4 °C,-20 °C

Stockage commentaire: Store the recombinant Histone H1 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Détails sur Histone H1

Antigène: Histone H1

Sujet: Please note that this antibody is a recombinant rabbit version of original anti-histone H1 antibody (clone AE-4). Because the variable heavy (VH) and variable light (VL) domains are the same, this recombinant antibody has the same exact reactivity as the original AE-4 MAb. There are several advantages of producing a recombinant version of a monoclonal antibody. For example, a recombinant antibody is a pure preparation of active immunoglobulin with no contaminating non-functional intact Ig or free light/heavy chains. Secondly, antibody can always be produced, even if hybridoma line is lost. Moreover, it adds the flexibility of converting the antibody to any species, isotype or format. Eukaryotic histones are basic and water-soluble nuclear proteins that form hetero-octameric nucleosome particles by wrapping 146 base pairs of DNA in a left-handed super-helical turn sequentially to form chromosomal fiber. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form the octamer, formed of two H2A-H2B dimers and two H3-H4 dimers, forming two nearly symmetrical halves by tertiary structure. Over 80 % of nucleosomes contain the linker Histone H1, derived from an intronless gene that interacts with linker DNA between nucleosomes and mediates compaction into higher order chromatin. This antibody is extensively used as a pan-nuclear marker.

UniProt: P07305