Keratin Basic anticorps

N° du produit ABIN7881441

Cet anticorps Souris Monoclonal détecte spécifiquement Keratin Basic dans IHC (p). Il présente une réactivité avec des échantillons de Humain.

Aperçu rapide pour Keratin Basic anticorps (ABIN7881441)

Antigène: Keratin Basic

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Inconjugué

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: SPM591

Détail du produit

Fonction: Basic Cytokeratin Antibody

Purification: Protein G affinity chromatography

Immunogène: Solubilized keratin extract from human stratum corneum was used as the immunogen for the Basic Cytokeratin antibody.

Isotype: IgG1, kappa

Information d'application

Indications d'application: Optimal dilution of the Basic Cytokeratin antibody should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10 mM Tris with 1 mM EDTA for 10-20 min followed by cooling at RT for 20 min.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: Prediluted in 1X PBS, 0.1 mg/mL BSA (US sourced), 0.05 % sodium azide, *For IHC use only*

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store the Basic Cytokeratin antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Détail du antigène

Antigène: Keratin Basic

Autre désignation: Basic Cytokeratin

Sujet: This antibody recognizes CK1, CK5, CK10 and CK14. In normal epithelia, it stains stratified epithelia, myoepithelial cells and basal cells in the prostate gland and bronchi. This mAb shows no reactivity with hepatocytes, pancreatic acinar cells, proximal renal tubules, or endometrial glands, there is no reactivity with cells derived from simple epithelia. Mesenchymal tumors, lymphomas, melanomas, neural tumors, and neuroendocrine tumors are negative with this antibody. It stains myoepithelial cells and has been shown to be useful in distinguishing prostate adenocarcinoma from benign prostate. This antibody has also been useful in separating benign from malignant intraductal breast proliferations.

UniProt: Q01546