MVP anticorps

N° du produit ABIN7881542

Cet anticorps anti-MVP Monoclonal Souris (Clone SPM280) (ABIN7881542) détecte spécifiquement MVP dans IHC (p). L’anticorps est réactif avec des échantillons de Humain.

Aperçu rapide pour MVP anticorps (ABIN7881542)

Antigène: MVP (Major Vault Protein (MVP))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp MVP est non-conjugé

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: SPM280

Détail du produit anti-MVP anticorps

Fonction: MVP Antibody

Purification: Protein G affinity chromatography

Immunogène: Proteins precipitated from human breast cancer MCF-7 cells were used as the immunogen for the MVP antibody.

Isotype: IgG1, kappa

Information d'application

Indications d'application: Optimal dilution of the MVP antibody should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris with 1 mM EDTA, pH 9, for 10-20 min followed by cooling at RT for 20 min.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: Prediluted in 1X PBS, 0.1 mg/mL BSA (US sourced), 0.05 % sodium azide, *For IHC use only*

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store the MVP antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Détails sur MVP

Antigène: MVP (Major Vault Protein (MVP))

Autre désignation: MVP

Sujet: Recognizes a protein of 104 kDa-110 kDa, characterized as major vault protein (MVP). Vaults are large ribonucleoprotein particles (RNPs) present in all eukaryotic cells. They have a complex morphology, including several small molecules of RNA, but a single protein species. The MVP accounts for >70 % of their mass. Their shape is reminiscent of the nucleopore central plug. Treatment of cells with estradiol increases the amount of MVP in nuclear extract. The hormone-dependent interaction of vaults with ER is prevented in vitro by sodium molybdate. Antibodies to estrogen, progesterone and glucocorticoid receptors are able to co-immunoprecipitate the MVP. MVP is overexpressed in many neoplastic tissues and cell lines. Expression of MVP predicts a poor response to chemotherapy.

UniProt: Q14764