NDST1 anticorps

N° du produit ABIN7881747

Cet anticorps Lapin Polyclonal détecte spécifiquement NDST1 dans WB et ELISA. Il présente une réactivité avec des échantillons de Humain, Souris et Rat.

Aperçu rapide pour NDST1 anticorps (ABIN7881747)

Antigène: NDST1 (N-Deacetylase/N-Sulfotransferase (Heparan Glucosaminyl) 1 (NDST1))

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp NDST1 est non-conjugé

Application: Western Blotting (WB), ELISA

Détail du produit anti-NDST1 anticorps

Fonction: NDST1 Antibody / N-deacetylase/N-sulfotransferase 1

Purification: Immunogen affinity purified

Immunogène: E.coli-derived human NDST1 recombinant protein (Position: 40-87) was used as the immunogen for the NDST1 antibody.

Isotype: IgG

Information d'application

Indications d'application: Optimal dilution of the NDST1 antibody should be determined by the researcher.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Stock: 4 °C,-20 °C

Stockage commentaire: After reconstitution, the NDST1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Détails sur NDST1

Antigène: NDST1 (N-Deacetylase/N-Sulfotransferase (Heparan Glucosaminyl) 1 (NDST1))

Autre désignation: NDST1

Sujet: NDST1 antibody detects N-deacetylase/N-sulfotransferase 1, an essential enzyme involved in the biosynthesis of heparan sulfate, a glycosaminoglycan that modulates cell signaling, adhesion, and extracellular matrix organization. NDST1 catalyzes two critical reactions-N-deacetylation and N-sulfation of glucosamine residues-forming the sulfated domains that give heparan sulfate its functional specificity. The NDST1 antibody is widely used in glycobiology, developmental, and cancer research to study proteoglycan biosynthesis, cell-matrix interactions, and signaling regulation.

NDST1 is encoded by the NDST1 gene located on human chromosome 5q33.1. The protein is approximately 882 amino acids long and resides in the lumen of the Golgi apparatus, where it acts early in heparan sulfate chain modification. NDST1 belongs to the heparan sulfate modification enzyme family, working in concert with other sulfotransferases and epimerases to establish specific sulfation patterns that control the binding affinity of heparan sulfate to growth factors, morphogens, and cytokines.

The NDST1 antibody detects a 100 kilodalton band by western blot and demonstrates perinuclear Golgi localization under immunofluorescence microscopy. NDST1 activity is essential for embryonic development, vascular formation, and neural patterning. Through its modification of heparan sulfate, NDST1 regulates key signaling pathways including FGF, Wnt, Hedgehog, and BMP. Loss or mutation of NDST1 disrupts heparan sulfate composition, leading to developmental abnormalities and impaired growth factor signaling.

In cancer, NDST1 expression is often dysregulated, resulting in altered extracellular matrix structure and aberrant cell signaling. Overexpression enhances tumor invasiveness by modifying cell-surface heparan sulfate, while reduced NDST1 activity interferes with angiogenesis. It also contributes to inflammation by modulating chemokine gradients and leukocyte migration.

Because NDST1 determines the sulfation code of heparan sulfate, it serves as a critical regulator of cell communication and extracellular architecture. NSJ Bioreagents provides a validated NDST1 antibody optimized for its applications, supporting investigations into glycosaminoglycan biosynthesis, developmental regulation, and tumor microenvironment signaling.

UniProt: P52848

Pathways: Regulation of Systemic Arterial Blood Pressure by Hormones, Glycosaminoglycan Metabolic Process