Recombinant NDUFB4 anticorps

N° du produit ABIN7881759

Cet anticorps Lapin Monoclonal détecte spécifiquement NDUFB4 dans WB, IF, IHC et ICC. Il présente une réactivité avec des échantillons de Humain, Rat et Souris.

Aperçu rapide pour Recombinant NDUFB4 anticorps (ABIN7881759)

Antigène: NDUFB4 (NADH Dehydrogenase (Ubiquinone) 1 beta Subcomplex, 4, 15kDa (NDUFB4))

Type d'anticorp: Recombinant Antibody

Reactivité: Humain, Rat, Souris

Hôte: Lapin

Clonalité: Monoclonal

Conjugué: Cet anticorp NDUFB4 est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunocytochemistry (ICC)

Clone: 30N45

Détail du produit anti-NDUFB4 anticorps

Fonction: NDUFB4 Antibody / NADH dehydrogenase ubiquinone 1 beta subcomplex subunit 4

Purification: Affinity chromatography

Immunogène: A synthesized peptide derived from human NDUFB4 was used as the immunogen for the NDUFB4 antibody.

Isotype: IgG

Information d'application

Indications d'application: Optimal dilution of the NDUFB4 antibody should be determined by the researcher.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: Rabbit IgG in phosphate buffered saline, pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol, 0.4-0.5 mg/mL BSA

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store the NDUFB4 antibody at -20oC.

Détails sur NDUFB4

Antigène: NDUFB4 (NADH Dehydrogenase (Ubiquinone) 1 beta Subcomplex, 4, 15kDa (NDUFB4))

Autre désignation: NDUFB4

Sujet: NDUFB4 antibody detects NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4, encoded by the NDUFB4 gene. This protein is a non catalytic accessory subunit of mitochondrial Complex I, the first enzyme complex of the oxidative phosphorylation system. Complex I catalyzes the transfer of electrons from NADH to ubiquinone, coupled with proton translocation across the inner mitochondrial membrane. NDUFB4 antibody provides researchers with an essential reagent for studying mitochondrial respiratory function, bioenergetics, and disease mechanisms.

Although NDUFB4 does not directly participate in electron transfer, it contributes to the structural stability and assembly of Complex I. Research using NDUFB4 antibody has shown that accessory subunits help maintain the integrity of the complex, ensuring efficient electron flow and proton pumping. Loss or dysfunction of accessory proteins like NDUFB4 destabilizes Complex I, leading to reduced oxidative phosphorylation and ATP production. This highlights the importance of even non catalytic subunits in respiratory chain function.

Mutations and defects in Complex I subunits, including NDUFB4, are associated with mitochondrial diseases such as Leigh syndrome, encephalopathy, and mitochondrial myopathy. Research using NDUFB4 antibody has demonstrated that absence or reduction of NDUFB4 impairs assembly of Complex I, leading to decreased activity and energy deficiency. Such dysfunctions contribute to neuromuscular symptoms, developmental delays, and metabolic acidosis. Because mitochondrial disease mechanisms are complex, NDUFB4 antibody serves as an important tool for dissecting assembly defects and functional outcomes.

In cancer, altered expression of Complex I subunits including NDUFB4 has been observed. Tumor cells often exhibit metabolic reprogramming, favoring glycolysis over oxidative phosphorylation. However, subsets of tumors rely on intact mitochondrial respiration, making Complex I subunits potential biomarkers and therapeutic targets. Studies with NDUFB4 antibody have shown correlations between expression levels and tumor progression, underscoring its relevance in oncology. Beyond cancer, mitochondrial dysfunction involving NDUFB4 contributes to neurodegenerative diseases, metabolic disorders, and aging related decline.

NDUFB4 antibody is applied in western blotting, immunohistochemistry, and immunofluorescence. Western blotting confirms protein abundance in mitochondria rich tissues such as brain, heart, and muscle. Immunohistochemistry highlights tissue distribution, while immunofluorescence demonstrates mitochondrial localization using colocalization with markers such as COX IV. These approaches ensure that NDUFB4 antibody is useful in both clinical and basic mitochondrial research.

By supplying validated NDUFB4 antibody reagents, NSJ Bioreagents supports research into oxidative phosphorylation, mitochondrial disease, and cancer biology. Detection of NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4 enables researchers to explore how Complex I subunits maintain cellular energy production and contribute to disease when impaired.

UniProt: O95168