Recombinant PKC anticorps

N° du produit ABIN7882075

L’anticorps anti-PKC Monoclonal Lapin est utilisé pour la détection de PKC dans des échantillons de Humain, Rat et Souris. Il a été validé pour WB, IF, ICC, FACS et IP.

Aperçu rapide pour Recombinant PKC anticorps (ABIN7882075)

Antigène: PKC (PKC pan) (Protein Kinase C (pan) (PKC pan))

Type d'anticorp: Recombinant Antibody

Reactivité: Humain, Rat, Souris

Hôte: Lapin

Clonalité: Monoclonal

Conjugué: Cet anticorp PKC est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunoprecipitation (IP)

Clone: 31P91

Détail du produit anti-PKC anticorps

Fonction: PKC Antibody / Protein kinase C

Purification: Affinity-chromatography

Immunogène: A synthesized peptide derived from human PKC was used as the immunogen for the PKC antibody.

Isotype: IgG

Information d'application

Indications d'application: Optimal dilution of the PKC antibody should be determined by the researcher.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: Rabbit IgG in phosphate buffered saline, pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol, 0.4-0.5 mg/mL BSA

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store the PKC antibody at -20oC.

Détails sur PKC

Antigène: PKC (PKC pan) (Protein Kinase C (pan) (PKC pan))

Autre désignation: PKC

Sujet: PKC antibody detects Protein kinase C (PKC), a family of serine/threonine kinases that play critical roles in cellular signal transduction, gene expression, and membrane receptor regulation. The UniProt recommended name is Protein kinase C. This enzyme family mediates diverse biological responses by phosphorylating target proteins involved in proliferation, apoptosis, differentiation, and cytoskeletal organization. PKC enzymes are key downstream effectors of diacylglycerol (DAG) and calcium signaling pathways, serving as central hubs that integrate extracellular signals into intracellular responses.

Protein kinase C includes multiple isoforms that can be broadly divided into three classes: conventional (alpha, beta, gamma), novel (delta, epsilon, eta, theta), and atypical (zeta, iota/lambda). Each isoform differs in its activation requirements and tissue distribution. Conventional isoforms require both calcium and DAG for activation, whereas novel isoforms are calcium-independent but DAG-dependent. Atypical isoforms do not require either calcium or DAG, responding instead to other lipid mediators. These differences allow PKC family members to finely tune signaling cascades in a context-dependent manner.

Functionally, PKC regulates numerous processes such as receptor desensitization, ion channel modulation, transcription factor activation, and cytoskeletal remodeling. It is activated by various stimuli including growth factors, hormones, and neurotransmitters through phospholipase C-mediated generation of DAG. Once activated, PKC translocates to cellular membranes where it phosphorylates specific substrates, leading to altered protein activity and gene expression. In neurons, PKC modulates synaptic plasticity, while in cardiac and smooth muscle it influences contraction and metabolism.

The PKC gene family is expressed across nearly all tissues, with particular enrichment in brain, heart, and skeletal muscle. Dysregulation of PKC activity has been linked to pathological conditions such as cancer, diabetes, neurodegeneration, and cardiovascular disease. In oncology, PKC can act as either a tumor promoter or suppressor depending on the isoform and cellular context. Therefore, antibodies targeting PKC are valuable tools for investigating its expression patterns, activation states, and downstream signaling pathways.

PKC antibody is useful in immunohistochemistry, immunofluorescence, and related assays to assess protein localization, phosphorylation-dependent signaling, and isoform-specific expression. By detecting total PKC or specific subtypes, researchers can study its role in signal transduction and disease progression. NSJ Bioreagents provides PKC antibody reagents optimized for research in kinase signaling, cellular communication, and phosphoregulation studies.

UniProt: P05129

Pathways: Positive Regulation of Response to DNA Damage Stimulus