Recombinant SAFB anticorps

N° du produit ABIN7882213

L’anticorps anti-SAFB Monoclonal Lapin est utilisé pour la détection de SAFB dans des échantillons de Humain, Souris et Rat. Il a été validé pour WB, IHC, IF et ICC.

Aperçu rapide pour Recombinant SAFB anticorps (ABIN7882213)

Antigène: SAFB (Scaffold Attachment Factor B (SAFB))

Type d'anticorp: Recombinant Antibody

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Monoclonal

Conjugué: Cet anticorp SAFB est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

Clone: 30S09

Détail du produit anti-SAFB anticorps

Fonction: SAFB Antibody / Scaffold attachment factor B

Purification: Affinity-chromatography

Immunogène: A synthesized peptide derived from human SAFB was used as the immunogen for the SAFB antibody.

Isotype: IgG

Information d'application

Indications d'application: Optimal dilution of the SAFB antibody should be determined by the researcher.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: Rabbit IgG in phosphate buffered saline, pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol, 0.4-0.5 mg/mL BSA

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store the SAFB antibody at -20oC.

Détails sur SAFB

Antigène: SAFB (Scaffold Attachment Factor B (SAFB))

Autre désignation: SAFB

Sujet: SAFB antibody detects scaffold attachment factor B, a multifunctional nuclear protein encoded by the SAFB gene. SAFB belongs to a family of proteins that bind scaffold/matrix attachment regions of DNA, thereby influencing chromatin structure and transcriptional regulation. It contains RNA recognition motifs and domains for protein-protein interactions, linking it to RNA processing, transcriptional repression, and nuclear architecture.

SAFB antibody is widely applied in molecular biology, transcriptional regulation, and cancer research. SAFB associates with nuclear matrix elements and modulates expression of hormone responsive genes. It also functions as a corepressor of estrogen receptor alpha, impacting signaling pathways critical in breast cancer biology. By detecting SAFB, researchers can explore mechanisms of gene regulation at the interface of DNA, RNA, and protein interactions.

Western blot assays detect SAFB protein in nuclear extracts, while immunohistochemistry maps expression in hormone responsive tissues and tumors. Immunofluorescence highlights nuclear speckle localization, consistent with its role in transcriptional complexes. These applications enable detailed characterization of SAFB across tissues and conditions.

SAFB also contributes to stress response pathways. It regulates heat shock protein gene expression and participates in apoptosis and cell cycle control. Mutations or altered expression of SAFB have been linked to breast cancer, prostate cancer, and neurological disorders. By applying SAFB antibody, scientists can evaluate its diverse functions in gene regulation, hormone signaling, and disease pathogenesis.

SAFB antibody from NSJ Bioreagents provides reliable specificity for analyzing nuclear scaffold interactions and transcriptional repression. Its performance across multiple assays ensures accurate results in both research and diagnostic contexts.

UniProt: Q15424

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway