CD43 anticorps

N° du produit ABIN7882270

L’anticorps anti-CD43 Monoclonal Souris est utilisé pour la détection de CD43 dans des échantillons de Humain. Il a été validé pour IHC (p).

Aperçu rapide pour CD43 anticorps (ABIN7882270)

Antigène: CD43 (SPN) (Sialophorin (SPN))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CD43 est non-conjugé

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: SPM503

Détail du produit anti-CD43 anticorps

Fonction: CD43 Antibody

Purification: Protein G affinity chromatography

Immunogène: Myeloblastic KG1 cells were used as the immunogen for this CD43 antibody.

Isotype: IgG1, kappa

Information d'application

Indications d'application: The optimal dilution of the CD43 antibody for each application should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10 mM Tris with 1 mM EDTA for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: Prediluted in 1X PBS, 0.1 mg/mL BSA (US sourced), 0.05 % sodium azide, *For IHC use only*

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store the CD43 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Détails sur CD43

Antigène: CD43 (SPN) (Sialophorin (SPN))

Autre désignation: CD43

Sujet: It recognizes a cell surface glycoprotein of 95/115/135 kDa (depending upon the extent of glycosylation), identified as CD43. 70-90 % of T-cell lymphomas and from 22-37 % of B-cell lymphomas express CD43. No reactivity has been observed with reactive B-cells. So a B-lineage population that co-expresses CD43 is highly likely to be a malignant lymphoma, especially a low-grade lymphoma, rather than a reactive B-cell population. When CD43 antibody is used in combination with anti-CD20, effective immunophenotyping of the lymphomas in formalin-fixed tissues can be obtained. Co-staining of a lymphoid infiltrate with anti-CD20 and anti-CD43 argues against a reactive process and favors a diagnosis of lymphoma.

UniProt: P16150

Pathways: Regulation of Leukocyte Mediated Immunity