Recombinant WDR4 anticorps

N° du produit ABIN7882696

L’anticorps anti-WDR4 Monoclonal Lapin est utilisé pour la détection de WDR4 dans des échantillons de Humain, Souris et Rat. Il a été validé pour WB, IHC, IF, FACS et ICC.

Aperçu rapide pour Recombinant WDR4 anticorps (ABIN7882696)

Antigène: WDR4 (WD Repeat Domain 4 (WDR4))

Type d'anticorp: Recombinant Antibody

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Monoclonal

Conjugué: Cet anticorp WDR4 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Immunocytochemistry (ICC)

Clone: 25W87

Détail du produit anti-WDR4 anticorps

Fonction: WDR4 Antibody / WD repeat domain 4

Purification: Affinity-chromatography

Immunogène: A synthesized peptide derived from human WDR4 was used as the immunogen for the WDR4 antibody.

Isotype: IgG

Information d'application

Indications d'application: Optimal dilution of the WDR4 antibody should be determined by the researcher.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: Rabbit IgG in phosphate buffered saline, pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol, 0.4-0.5 mg/mL BSA

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store the WDR4 antibody at -20oC.

Détails sur WDR4

Antigène: WDR4 (WD Repeat Domain 4 (WDR4))

Autre désignation: WDR4

Sujet: WDR4 antibody detects WD repeat domain 4, a conserved protein involved in RNA modification and tRNA methylation. WDR4 forms a complex with METTL1 to catalyze the N7-methylguanosine modification of tRNAs, a modification required for translational fidelity and RNA stability. The WDR4 antibody is widely used in molecular biology and RNA research to study RNA methylation, protein translation, and gene expression regulation.

WDR4 is encoded by the WDR4 gene located on human chromosome 21q22.3. The protein is approximately 412 amino acids long and contains several WD40 repeats that mediate protein-protein interactions, forming a beta-propeller structure typical of the WD repeat family. WDR4 localizes to the nucleus and cytoplasm, where it partners with METTL1 to catalyze methylation of specific tRNA guanine residues, ensuring proper translation initiation and elongation.

The WDR4 antibody detects a 46 kilodalton protein by western blot and demonstrates nuclear and cytoplasmic staining patterns under immunofluorescence. Through its interaction with METTL1, WDR4 stabilizes the tRNA methyltransferase complex and regulates global protein synthesis. Loss of WDR4 leads to reduced tRNA methylation, impaired translation, and increased sensitivity to stress.

Mutations in WDR4 cause a spectrum of developmental and neurological disorders, including microcephaly and intellectual disability. These defects arise from disrupted tRNA modification, which affects the translation of growth-related and neuronal genes. In cancer, overexpression of WDR4 enhances translation of oncogenic proteins and promotes tumor progression through metabolic reprogramming.

Because of its key role in RNA modification and translation regulation, WDR4 serves as an essential model for understanding epitranscriptomic control of gene expression. NSJ Bioreagents provides a validated WDR4 antibody optimized for its applications, supporting studies of RNA methylation, translational regulation, and disease pathogenesis.

UniProt: P57081