CEA anticorps

N° du produit ABIN7884237

Cet anticorps Souris Monoclonal détecte spécifiquement CEA dans IHC et ELISA. Il présente une réactivité avec des échantillons de Humain.

Aperçu rapide pour CEA anticorps (ABIN7884237)

Antigène: CEA (Carcinoembryonic Antigen Gene Family (CEA))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CEA est non-conjugé

Application: Immunohistochemistry (IHC), ELISA

Clone: C234

Détail du produit anti-CEA anticorps

Fonction: Carcinoembryonic antigen (CEA) Mouse Monoclonal Antibody (C234)

Specificité: This antibody is CEA specific. It does not recognize NCA, neither in enzyme linked immunosorbent assay (ELISA) not on Western blots of crude tissue extracts. This antibody binds to CEA producing human adenocarcinoma cells (e.g. LS174T,WiDr) but does not bind to CEA non-secreting human adenocarcinoma cells (Colo 320DM). It does not immunostain cryocuts of any normal human tissues tested (artery, heart muscle, kidney, large bowel, liver, lung, skeletal muscle, skin, small gut, spleen, testes or vein) neither does it react with any antigens on human blood cells when separated by fluoresceine actuated cell sorter (FACS). Immunohistochemical analysis of cancerous tissues revealed that the antibody bound selectively adenocarcinoma and not carcinoid tumor of the colon, as well as tumor- associated CEA in breast, gastric and lung neoplasms, while there was no immunohistochemical evidence of cross-reactivity with NCA. A remarkable feature of this antibody is its ability to distinguish immunohistochemically between cancer and residual normal tissue within the same organ.

Purification: Purified

Immunogène: Physically modified CEA

Isotype: IgG kappa

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Reconstitute with deionized water. Optimal dilution has to be determined by the user.

Buffer: Lyophilized Protein A purified in PBS pH 7.4 (Full IgG)

Agent conservateur: Without preservative

Stock: 4 °C,-20 °C,-80 °C

Stockage commentaire: Lyophilized antibodies can be kept at 4°C for up to 3 months and should be kept at -20°C for long-term storage (2 years). To avoid freeze-thaw cycles, reconstituted antibodies should be aliquoted before freezing for long-term (1 year) storage (-80°C) or kept at 4°C for short-term usage (2 months). For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made with the assay buffer. After the maximum long-term storage period (2 years lyophilized or 1 year reconstituted) antibodies should be tested in your assay with a standard sample to verify if you have noticed any decrease in their efficacy. To limit antibody loss or degradation, BSA (final concentration 1%) and sodium azide (final concentration 0.02%) can be added to the suggested first dilution. It is important to first verify if those preservatives are compatible with your assay.

Date de péremption: 24 months

Détails sur CEA

Antigène: CEA (Carcinoembryonic Antigen Gene Family (CEA))

Autre désignation: Carcinoembryonic antigen (CEA)

Sujet:

CEA

Carcinoembronic antigen (CEA), an oncodevelopmental tumor marker is a fairly typical, high-molecular weight, cell surface glycoprotein over-expressed in adenocarcinomas of entero-dermally-derived digestive system epithelia and in fetal colon. The majority of antibodies induced against CEA would cross-react with a variety of proteins present in normal tissues such as non-specific cross-reacting antigen (NCA). Highly specific anti-CEA mouse monoclonal antibody was produced as a result of a unique imunization protocol.