GPR4 anticorps (Extracellular Loop)

N° du produit ABIN7884985

L’anticorps anti-GPR4 Polyclonal Lapin est utilisé pour la détection de GPR4 dans des échantillons de Humain, Rat et Souris. Il a été validé pour WB et IHC.

Aperçu rapide pour GPR4 anticorps (Extracellular Loop) (ABIN7884985)

Antigène: GPR4 (G Protein-Coupled Receptor 4 (GPR4))

Reactivité: Humain, Rat, Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp GPR4 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Classe de qualité: Carrier-free

Détail du produit anti-GPR4 anticorps

Épitope: AA 162-177, Extracellular Loop

Fonction: A Rabbit Polyclonal Antibody to GPR4

Homologie: Rat,mouse,human - 15,16 amino acid residues identical.

Purification: Affinity purified on immobilized antigen.

Immunogène: (C)RDRYNHTF(S)FEKFPME, corresponding to amino acid residues 162-177 of rat GPR4

Isotype: IgG

Information d'application

Indications d'application:

WB: 1:200

FC: 2.5 μg per reaction

ICC: The optimal concentration should be determined by the user

IHC: 1:400

IP: The optimal concentration should be determined by the user

Commentaires:

Negative Control: (ABIN7235415)

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Agent conservateur: Without preservative

Stock: -20 °C

Stockage commentaire: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Détails sur GPR4

Antigène: GPR4 (G Protein-Coupled Receptor 4 (GPR4))

Autre désignation: GPR4

Sujet:

Synonyms: Acid-sensing G-protein coupled receptor 4, GPR19

Description: G-protein coupled receptor 4 (GPR4) belongs to a protein family comprised of 3 related G-protein coupled receptors (GPCRs): GPR4, OGR1/GPR68 and TDAG8/GPR65. Like all GPCRs, GPR4's structure is composed of seven-transmembrane-spanning domains linked by an N-terminal extracellular domain and a C-terminal intracellular domain2.GPR4 responds to pH changes and is activated by a physiological increase in extracellular H+ through several signaling pathways including- the G12/13-protein/Rho, the Gq/PLC, and the Gs-protein/cAMP pathway1,2. It is fully activated at pH levels of 6.8 and lower and is inactive at pH levels of 7.5 and higher3,4.Recent studies show that the pH -sensing GPCRs also have the ability to regulate cancer cell metastasis and proliferation, immune cell function, inflammation, blood vessel formation, endothelial barrier function thus making them an important therapeutic target for different diseases. Overexpression of GPR4 in human cancer cells shows involvement in driving or maintaining tumor formation.GPR4 is expressed in several tissues including lung, vascular endothelial cells, kidney, heart, liver, skeletal muscle, ovary, and placenta.

ID gène: 308408

UniProt: Q4KLH9