GPR65 anticorps (Extracellular Loop)

N° du produit ABIN7884991

L’anticorps anti-GPR65 Polyclonal Lapin est utilisé pour la détection de GPR65 dans des échantillons de Humain, Souris et Rat. Il a été validé pour FACS, LCI et WB.

Aperçu rapide pour GPR65 anticorps (Extracellular Loop) (ABIN7884991)

Antigène: GPR65 (G Protein-Coupled Receptor 65 (GPR65))

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp GPR65 est non-conjugé

Application: Flow Cytometry (FACS), Live Cell Imaging (LCI), Western Blotting (WB)

Classe de qualité: Carrier-free

Détail du produit anti-GPR65 anticorps

Épitope: AA 72-84, Extracellular Loop

Fonction: A Rabbit Polyclonal Antibody to GPR68 (OGR1)

Homologie: Rat,mouse,human - 14,15 amino acid residues identical.

Purification: Affinity purified on immobilized antigen.

Immunogène: (C)DYTWNKDNWTFSP, corresponding to amino acid residues 72 - 84 of human GPR65

Isotype: IgG

Information d'application

Indications d'application:

WB: 1:200

FC: 2.5 μg per reaction

ICC: The optimal concentration should be determined by the user

IHC: The optimal concentration should be determined by the user

IP: The optimal concentration should be determined by the user

Commentaires:

Negative Control: (ABIN7235434)

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW)

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Agent conservateur: Without preservative

Stock: -20 °C

Stockage commentaire: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Détails sur GPR65

Antigène: GPR65 (G Protein-Coupled Receptor 65 (GPR65))

Autre désignation: G-protein coupled receptor 65

Sujet:

Synonyms: Acid-sensing G-protein coupled receptor 68, Ovarian cancer G-protein coupled receptor 1, Sphingosylphosphorylcholine receptor

Description: All GPCRs, including GPR68, are single peptides possessing seven transmembrane domains with an extracellular N-terminus and an intracellular C-terminus1. GPR68 is expressed in several tissues including the spleen, testis, small bowel, brain, heart, lung and kidneys. It shares strong homology (49-54 % ) with the human receptor GPR42.GPR68 is proton sensitive in the pH range of 6.8-7.8 and acts as a proton activated receptor. GPR68 stimulates inositol triphosphate (IP3) formation thereby releasing intracellular calcium and activating the mitogen activated protein kinase/extracellular signal-regulated (ERK) kinase cascade. In addition, GPR68 receptor regulates NHE3 and H+-ATPase, in response to extracellular pH changes in cultured HEK 293 renal cells. This activation requires a cluster of histidine residues in the extracellular domains of the receptor and protein-kinase C (PKC) activation4.

ID gène: 238377

UniProt: Q8BFQ3

Pathways: cAMP Metabolic Process